DEVELOPMENT OF AN IMPROVED SMALL DIAMETER VASCULAR GRAFT

改进的小直径血管移植物的开发

• 批准号: 3501114
• 负责人: PAUL T PICCIANO
• 金额: $ 4.64万
• 依托单位: BIOPOLYMERS, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1987-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3501114
• 关键词: biomaterial development /preparation; blood vessel prosthesis; fluorocarbon polymers; surgery material /equipment; tissue /cell culture; vascular endothelium

For over thirty years, a major goal of vascular surgery has been to improve bypass surgery through the development of an ideal prosthetic graft. Although saphenous vein is the preferred substrate for occluded small to medium veins, frequently it is unavailable or unsuitable to a great number of patients who need this surgery. Two of the problems that have impaired the development of such a graft include poor cell attachment upon seeding grafts and poor retention of cells by grafts when exposed to in vitro or in vivo flow conditions. Although the use of fibronectin has resulted in some improvements with respect to these parameters, development of grafts is still far from complete. This proposal describes experiments on mussel adhesive protein (MAP, BioPolymers, Inc. Farmington, CT). This protein has the potential to increase the adhesion of cells to grafts in vitro and in vivo. MAP has been found to be effective as a cell attachment factor in vitro and is biocompatible with cells and tissues. Experiments are designed to explore attachment efficiency of cells to grafts and the retention of these cells by grafts during perfusion.

三十多年来，血管外科的一个主要目标是 通过开发一种理想来改进搭桥手术 假体移植。虽然大隐静脉是首选 用于闭塞的中小静脉的衬底，通常是 无法或不适合大量有需要的患者 这次手术。两个问题损害了 这种移植物的发展包括细胞在 种植移植物，暴露时移植物对细胞的保持性差 达到体外或体内流动的条件。虽然使用了 纤维连接蛋白在以下方面有了一些改善 这些参数，移植物的发展还远远没有 完成。 介绍了贻贝黏附蛋白的实验研究。 (MAP，生物聚合物公司，康涅狄格州法明顿)这种蛋白质具有 在体外和体内提高细胞与移植物的粘附性的可能性 活着。已发现MAP作为细胞附件是有效的 在体外是因子，与细胞和组织具有生物相容性。 实验旨在探索附着力的效率 细胞到移植物和移植物对这些细胞的保留 灌流。