PARATHYROID IMAGING BY RADIOLABELED MONOCLONAL ANTIBODY

使用放射性标记单克隆抗体进行甲状旁腺成像

• 批准号: 3491452
• 负责人: CHARLES V BENTON
• 金额: $ 5万
• 依托单位: INVITRON CORPORATION
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3491452
• 关键词: antibody specificity; antigens; chemical synthesis; endocrine disorder diagnosis; flow cytometry; hybridomas; imaging /visualization /scanning; immunoglobulin G; immunoglobulin M; immunotherapy; monoclonal antibody; neoplasm /cancer radionuclide diagnosis; parathyroid gland; radionuclide diagnosis; radiopharmacology; radiotracer

The goal of this project is to develop a monoclonal antibody-based imaging reagent capable of visualizing human parathyroid glands. Phase I studies will be directed to isolation and characterization of human parathyroid-specific mouse monoclonal antibodies and preliminary analysis of their target antigens. Secondly, because the class and sub-class of antibodies will likely influence imaging properties, variants of hybridomas will be selected by fluorescence activated cell sorter that secrete immunoglobulins of different IgG subclasses and IgM. These antibodies will be tested for localization of human parathyroid fragments in nude mice. Phase II studies will likely focus on the imaging parameters of these variant antibodies in large animals, such as dogs or monkeys, to select the optimal conditions for imaging in humans.

该项目的目标是开发一种基于单抗的成像技术。 能使人甲状旁腺显影的试剂。第一阶段研究 将针对人类的隔离和特征 甲状旁腺特异性鼠单抗及其初步分析 它们的目标抗原。其次，因为类和子类的 抗体可能会影响杂交瘤的成像特性和变种 将通过分泌的荧光激活细胞分选器进行选择 不同Ig G亚类和Ig M免疫球蛋白。这些抗体将 在裸鼠体内检测人甲状旁腺片段的定位。 第二阶段的研究可能会集中在这些成像参数上 大型动物(如狗或猴子)中的变异抗体，以选择 人体成像的最佳条件。