A CRISPRi screen of essential genes of Neisseria gonorrhoeae
A CRISPRi screen of essential genes of Neisseria gonorrhoeae
批准号:
10656956
负责人:
Hank S. SEIFERT
金额:
$24.0万
依托单位国家:
美国
项目类别:
财政年份:
2023
资助国家:
美国
项目状态:
未结题
起止时间:
2023-07-21 至 2025-06-30
关键词:
AnatomyAntibiotic ResistanceBacteriaCRISPR interferenceCRISPR libraryCaliforniaCase StudyClustered Regularly Interspaced Short Palindromic RepeatsCommunitiesCompetenceComplementConsensusDNADevelopmentDiseaseEssential GenesFundingGene Expression RegulationGene OrderGenesGeneticGonorrheaGrantHumanIndividualInfectionInternationalInvestigationKnock-outKnowledgeLaboratoriesLettersLibrariesMichiganModern 1601-historyMutateNeisseriaNeisseria gonorrhoeaeNeisseria lactamicaNeisseria meningitidisOpen Reading FramesOrganismPathogenesisPathogenicityPhylogenetic AnalysisPhysiologyPlasmidsPopulationR24ReportingResearchResearch PersonnelResource SharingResourcesSexually Transmitted AgentsSexually Transmitted DiseasesSuggestionSystemTechnologyUncertaintyUniversitiesVaccinesWorkantimicrobialconditional mutantdrug-resistant gonorrheafundamental researchgenetic resourcehuman pathogeninnovationknockout genemutantpathogenresistance mechanismsymposiumsynthetic constructtransmission processvaccine candidate
中文摘要
摘要
英文摘要
Abstract
Neisseria gonorrhoeae is the sole causative agent of the sexually transmitted infection gonorrhea that
only lives in the human population. N. gonorrhoeae has co-evolved with its human host throughout
modern history and has evolved specific mechanisms of pathogenesis that relate to its mode of
transmission and the anatomical sites it colonizes. Rates of gonorrhea are rising along with the rapid
acquisition of antibiotic resistance. There is an urgent need to understand the mechanisms of N.
gonorrhoeae pathogenesis to develop new treatment options or vaccine candidates. N. gonorrhoeae
is naturally competent for DNA transformation and is one of a few bacterial species that does not
regulate competence. This grant will use CRISPR interference technology (CRISPRi) to determine
the essential genes of N. gonorrhoeae and provide the research community with the ability to regulate
the essential genes. This work will complement our funded R24 resource grant, which creates
insertional mutants of every open reading frame of N. gonorrhoeae but cannot mutate essential
genes. The R24 project is leveraging the transformation competence of N. gonorrhoeae and synthetic
DNA constructs to create ordered gene knockout libraries of the two most used N. gonorrhoeae
strains, MS11 and FA1090. These strains represent different phylogenetic clades of the bacterium.
With this conditional mutant library of CRISPRi, researchers can interrogate the entire N.
gonorrhoeae gene content to advance fundamental research and vaccine or antimicrobial
development.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
29th Annual Midwest Microbial Pathogenesis Conference (MMPC)
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批准号:10750604
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项目类别:
-
资助金额:$0.95万
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财政年份:2023
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负责人:Hank S. SEIFERT
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依托单位:
Ordered gene knockout libraries for Neisseria gonorrhoeae
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批准号:10088802
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项目类别:
-
资助金额:$77.55万
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财政年份:2021
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负责人:Hank S. SEIFERT
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依托单位:
Ordered gene knockout libraries for Neisseria gonorrhoeae
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批准号:10437587
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项目类别:
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资助金额:$77.71万
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财政年份:2021
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负责人:Hank S. SEIFERT
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依托单位:
Ordered gene knockout libraries for Neisseria gonorrhoeae
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批准号:10649487
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项目类别:
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资助金额:$61.71万
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财政年份:2021
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负责人:Hank S. SEIFERT
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依托单位:
Targeting the functions of the gonococcal Type lV pilus
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批准号:10663812
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项目类别:
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资助金额:$56.29万
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财政年份:2020
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负责人:Hank S. SEIFERT
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依托单位:
Mechanisms of Gonococcal Pilin Antigenic and Phase Variation
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批准号:10116928
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项目类别:
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资助金额:$3.95万
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财政年份:2020
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负责人:Hank S. SEIFERT
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依托单位:
Targeting the functions of the gonococcal Type lV pilus
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批准号:10451717
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项目类别:
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资助金额:$53.3万
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财政年份:2020
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负责人:Hank S. SEIFERT
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依托单位:
An unbiased screen to identify Neisseria gonorrhoeae genes important for survival to neutrophil killing mechanisms
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批准号:10058123
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项目类别:
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资助金额:$15.8万
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财政年份:2020
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负责人:Hank S. SEIFERT
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依托单位:
An unbiased screen to identify Neisseria gonorrhoeae genes important for survival to neutrophil killing mechanisms
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批准号:10197033
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项目类别:
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资助金额:$27.91万
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财政年份:2020
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负责人:Hank S. SEIFERT
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依托单位:
Targeting the functions of the gonococcal Type lV pilus
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批准号:10224790
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项目类别:
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资助金额:$48.14万
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财政年份:2020
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负责人:Hank S. SEIFERT
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依托单位:
Gonococcal Pilus Structure and Function
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批准号:6844919
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项目类别:
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资助金额:$29.7万
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财政年份:2004
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负责人:Hank S. SEIFERT
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依托单位:
Gonococcal Pilus Structure and Function
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批准号:6773647
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项目类别:
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资助金额:$29.7万
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财政年份:2004
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负责人:Hank S. SEIFERT
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依托单位:
Gonococcal Pilus Structure and Function
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批准号:7009073
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项目类别:
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资助金额:$29.0万
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财政年份:2004
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负责人:Hank S. SEIFERT
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依托单位:
Gonococcal Pilus Structure and Function
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批准号:7340382
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项目类别:
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资助金额:$27.63万
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财政年份:2004
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负责人:Hank S. SEIFERT
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依托单位:
Gonococcal Pilus Structure and Function
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批准号:7173342
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项目类别:
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资助金额:$28.16万
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财政年份:2004
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负责人:Hank S. SEIFERT
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依托单位:
Molecular genetics of the gonococcus
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批准号:7541427
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项目类别:
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资助金额:$27.63万
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财政年份:1998
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负责人:Hank S. SEIFERT
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依托单位:
MOLECULAR ANALYSIS OF GONOCOCCAL RECA FUNCTIONS
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批准号:6328809
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项目类别:
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资助金额:$17.47万
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财政年份:1998
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负责人:Hank S. SEIFERT
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依托单位:
Molecular Genetics of the gonococcus
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批准号:6721969
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项目类别:
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资助金额:$25.99万
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财政年份:1998
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负责人:Hank S. SEIFERT
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依托单位:
Molecular Genetics of the Gonococcus
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批准号:8434171
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项目类别:
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资助金额:$35.48万
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财政年份:1998
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负责人:Hank S. SEIFERT
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依托单位:
Molecular Genetics of the Gonococcus
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批准号:8640068
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项目类别:
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资助金额:$37.74万
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财政年份:1998
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负责人:Hank S. SEIFERT
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依托单位:
海外基金