BIOCHEMICAL MODEL REACTIONS

生化模型反应

• 批准号: 3484257
• 负责人: RONALD BRESLOW
• 金额: $ 31.99万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-01-01 至 1990-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3484257
• 关键词: Diels Alder reaction; alcohol dehydrogenase; aminoacid biosynthesis; carboxypeptidase; chemical binding; chemical models; chemical synthesis; cofactor; cyclization; cyclodextrins; electrochemistry; enzyme model; hydrolase; lysozyme; metalloenzyme; molecular site; nutrition related tag; oxidoreductase; pancreatic ribonuclease; stereochemistry; steroids; transaminases; tryptophan synthase; vitamin B6

Work on artificial enzymes will be pursued. This includes compounds combining cyclodextrins, pyridoxal or pyridoxamine with binding groups, such as cyclodextrins, macrocycles, or steroids; appropriate placement of basic catalytic groups will also be used to promote specific processes. The characteristic specificities of enzyme reactions--for substrate, for chemical reaction, for region of the substrate, and for stereochemistry of substrate and produce--will be mimicked. Work on thiamine related enzyme mimics will also be pursued, as will work on the synthesis and study of the hydrophobic cage molecules. Design of these syst;ems will be assisted by computer modeling methods. Work will be pursued on the design of catalysts that cleave RNA, using a newly developed assay technique. Studies of the catalysis of Diels-Alder reactions and related processes by hydrophobic effects and cage catalysts will be pursued, emphasizing selectivity. Work will also be directed at producing good mimics of peptide-cleaving and synthesizing enzymes.

将继续开展人工酶的研究。 这包括化合物 将环糊精、吡哆醛或吡哆胺与结合基团组合， 例如环糊精、大环化合物或类固醇；适当放置 碱性催化基团也将用于促进特定过程。 酶反应的特征——对于底物，对于 化学反应，对于底物区域，以及对于立体化学 基质和产品将被模仿。 硫胺素相关酶的研究工作 还将追求模仿，并将致力于合成和研究 疏水笼分子。 这些系统的设计将得到以下人员的协助 计算机建模方法。 将开展催化剂设计工作 使用新开发的检测技术来切割 RNA。 研究的 疏水性狄尔斯-阿尔德反应及相关过程的催化 将追求效应和笼式催化剂，强调选择性。 工作 还将致力于生产肽切割的良好模拟物和 合成酶。