STRUCTURAL KINETIC STUDIES BY NUCLEAR MAGNETIC RESONANCE

核磁共振结构动力学研究

• 批准号: 3484348
• 负责人: ALFRED Guillou REDFIELD
• 金额: $ 18.18万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3484348
• 关键词: allosteric site; aminoacid tRNA ligase; bacteria; bacterial RNA; carbon; chemical reaction; conformation; enzyme complex; enzyme structure; microorganism culture; molecular site; nitrogen; nuclear magnetic resonance spectroscopy; nucleic acid structure; radiotracer; stable isotope; transfer RNA

NMR Spectroscopy will continue to be applied to determine the structure and dynamics of transfer RNA in interaction with the enzyme aminoacyl tRNA synthetase. These two macromolecules are essential parts of the biosynthetic machinery of all living things. The emphasis is on use of isotope labels of 15N and 13C to highlight proton NMR signals and provide more information than straight NMR spectroscopy. Smaller molecules interacting with the enzyme, as well as labelled enzyme and labelled tRNA, will be examined to provide information on molecular changes during function of the molecules. The NMR methods being used are prototypical of methods potentially applicable to drug design.

核磁共振波谱将继续用于确定结构和 转移RNA与氨酰tRNA酶相互作用的动力学研究 合成酶。这两个大分子是 所有生物的生物合成机械。重点是使用 15N和13C的同位素标记以突出质子核磁共振信号并提供 比直接的核磁共振波谱提供更多的信息。小分子 与酶以及标记的酶和标记的tRNA相互作用， 将被检查以提供有关分子变化的信息 分子的功能。正在使用的核磁共振方法是典型的 可能适用于药物设计的方法。