STRUCTURAL KINETIC STUDIES BY NUCLEAR MAGNETIC RESONANCE

核磁共振结构动力学研究

• 批准号: 3269919
• 负责人: ALFRED Guillou REDFIELD
• 金额: $ 19.51万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-01 至 1986-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3269919
• 关键词: allosteric site; amides; bacteria; bacterial RNA; chemical reaction; conformation; enzyme complex; enzyme structure; hydrogen transport; ion transport; ligase; microorganism culture; molecular rearrangement; nuclear magnetic resonance spectroscopy; nucleic acid structure; transfer RNA; water solution

Nuclear magnetic resonance is being applied to the study of transfer RNA, and of related proteins. Current emphasis is on identification, structure, and dynamic behavior of tRNA. Identification and structure are being approached mainly by nuclear Overhauser effect, as well as studies of carefully chosen different tRNA species. Dynamic behavior is being inferred mainly from studies of solvent exchange rates, measured by line broadening, by relaxation-time measurement, and by rapid change of solvent from H2O to D2O.

核磁共振正被应用于转移RNA的研究，并 相关蛋白质。当前的重点是识别、结构和动态 TRNA的行为。识别和结构主要是通过以下方式进行的 核Overhauser效应，以及精心选择的不同tRNA的研究 物种。动力学行为主要是从溶剂的研究中推断出来的 汇率，通过线条加宽测量，通过放松时间测量，以及 通过迅速将溶剂从H2O转变为D2O。