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Usefulness of radioimmunodetection and radioimmunotherapy with polymonoclonal antitumor antibody using melanoma bearing mice.

使用携带黑色素瘤的小鼠进行放射免疫检测和使用多单克隆抗肿瘤抗体进行放射免疫治疗的有用性。

基本信息

批准号：
63570498
负责人：
KUSAKABE Kiyoko
金额：
$ 1.34万
依托单位：
Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

This study is to prove the different biodistributions in the tumor using two kinds of antimelanoma antibodies in Vivo.Although scintigraphy with radioisotope labeled monoclonal antibodies to image the tumor has proved to be useful, the localization of the tumor was not always successful, because monoclonal antibodies binds only the limited cells within the tumor.This study was undertaken in the different distributions of antimelanoma antibodies (AMAb),in the mouse melanoma.Two species of murine syngeneic monoclonal AMAb (M562 and M2590; IgM) have been established at our institutions. These monoclonal antibodies were obtained by fusion of BALB/C myeloma and C57BL/6 spleen cells primed with syngeneic B16 melanoma cells. M2590 was reacted to both human and mouse melanomas. M562 showed no reaction for any tumors except B16 mouse melanoma.This initial findings showed that radioisotope labeled monoclonal AMAb was accumulated in melanoma bearing mice specifically, and that these antibodies were distributed inhomogeneously on each macroautoradiogram in the tumors. About 3.7 MBq of 1-131 labeled antibody arid 1.11 MBq of 1-125 labeled antibody were injected into the mice bearing syngeneic mouse melanoma. The distribution of 1-131 labeled antibody was obtained on film within one week after the preparation of mouse specimen. About three months later the different distributions of the mouse AMAb were observed on autoradiogram using the double nuclide technique.The result suggests that preferential accumulations of two different AMAbs in the tumors were owing to the antibodies directed against a determinant on cell surface. The polymonoclonal antibodies, therefore, can be given to nigher tumor uptakes and also useful for radioimmutiodetection and radioimmnunotherapy.

本研究旨在证明两种抗黑色素瘤抗体在肿瘤中的不同生物分布。虽然放射性同位素标记的单克隆抗体对肿瘤成像已被证明是有用的，但肿瘤定位并不总是成功的，因为单克隆抗体仅结合肿瘤内有限的细胞。本研究在抗黑色素瘤抗体（AMAb）的不同分布中进行，在我们的研究所已经建立了两种小鼠同基因单克隆AMAb（M562和M2590; IgM）。这些单克隆抗体是通过BALB/C骨髓瘤细胞与用同基因B16黑色素瘤细胞致敏的C57 BL/6脾细胞融合获得的。M2590对人和小鼠黑色素瘤均具有反应性。M562对除B16小鼠黑色素瘤外的其他肿瘤均无反应，初步结果表明，放射性同位素标记的单克隆AMAb在荷黑色素瘤小鼠体内特异性蓄积，并且这些抗体在肿瘤的每个宏观放射自显影图上分布不均匀。将约3.7MBq的1-131标记抗体和1.11MBq的1-125标记抗体注射到携带同基因小鼠黑色素瘤的小鼠中。在制备小鼠标本后一周内，在胶片上获得1-131标记抗体的分布。约3个月后，用双核素放射自显影技术观察到两种AMAb在肿瘤中的不同分布，结果提示两种AMAb在肿瘤中的优先聚集是由于针对细胞表面决定簇的抗体所致。因此，多克隆抗体可用于更高的肿瘤摄取，并可用于放射免疫检测和放射免疫治疗。