NOVEL IMMUNOGENS FOR AMPHETAMINE ENZYME IMMUNOASSAY

用于安非他明酶免疫测定的新型免疫原

• 批准号: 3494283
• 负责人: JOHN M FRAZER
• 金额: $ 4.9万
• 依托单位: GENETIC DIAGNOSTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3494283
• 关键词: amphetamines; antibody specificity; diagnosis design /evaluation; drug abuse; enzyme linked immunosorbent assay; hybridomas; laboratory mouse; methamphetamine; monoclonal antibody; tissue /cell culture

Drug abuse has created a continuing need for a rapid and accurate system for the detection of amphetamines. This SBIR proposal addresses the need for a rapid and specific enzyme-linked immunosorbent assay (ELISA) for amphetamines which can be performed with only modest laboratory facilities. Since the critical componet of any immunoassay is the affinity and specificity of the antibody used, we propose to generate specific monoclonal antibodies for both amphetamine and methamphetamine which do not detect very similar compounds such as phenylpropanolamine, ephedrine, and phentermine which are present in many over the counter cold, allergy and diet medications. Our specific goals during the Phase I period will be to prepare several "sterically rigid" amphetamine immunogens, and use them to generate monoclonal antibodies by standard hybridoma procedures. We will characterize these antibodies for affinity for amphetamine/methamphetamine and cross reactivity with phenylpropanolamine, ephedrine, and phentermine. We will also investigate the new monoclonal antibodies for use in polystyrene microtiter plate ELISA systems, which we have been developing for other drugs of abuse including benzodiazepines, cocaine, and THC.

药物滥用造成了对迅速和准确的治疗的持续需要