DETECTION OF PASSIVE SMOKING-A NOVEL ELISA FOR COTININE

被动吸烟的检测——可替宁的新型酶联免疫吸附试验

• 批准号: 3501872
• 负责人: JOHN M FRAZER
• 金额: $ 3.02万
• 依托单位: SEREX
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3501872
• 关键词: catalase; chemical conjugate; enzyme linked immunosorbent assay; glucose oxidase; nicotine; passive smoking; peptide chemical synthesis; peroxidases

Tobacco smoke continues to be cited as the causative agent in a wide number of human diseases and pathologies. The dangers from tobacco smoke have been extended to include health problems derived from the passive inhalation of tobacco smoke byu non-smokers. This SBIR proposal addresses the need for a rapid, sensitive, and specific enzyme-linked immunosorbent assay for cotinine, the major metabolite of nicotine, which can be performed by physicians in the field. We propose to modify our current, commercially available ELISA for cotinine by developing a novel assay format so that the assay an be performed without instrumentation for a clear discrimination between non-smokers, non-smokers passively exposed to tobacco smoke, and active smokers. Our specific goals are to generate new enzyme-antibody conjugates and new antigen-enzyme conjugates and put them in an assay format which will generate a color in the presence of continue at levels of 5 to 500 ng/ml and remain colorless in the absence of cotinine.

烟草烟雾继续被认为是许多疾病的病原体， 人类疾病和病理学。 烟草烟雾的危害 被扩展到包括来自被动的健康问题， 不吸烟者吸入烟草烟雾。 该SBIR提案涉及 需要一种快速、灵敏和特异的酶联免疫吸附剂 可替宁（尼古丁的主要代谢物）的测定，可以 由该领域的医生进行。 我们建议修改我们目前的， 可替宁的市售ELISA 格式，以便可以在没有仪器的情况下进行测定， 明确区分不吸烟者，不吸烟者被动暴露于 烟草烟雾和积极吸烟者。 我们的具体目标是产生新的酶-抗体缀合物和新的 抗原-酶缀合物，并将它们置于测定形式中， 在浓度为5 - 500 ng/ml时产生颜色 并且在没有可替宁的情况下保持无色。