MEASUREMENT OF IN VIVO CEREBRAL METABOLISM BY C13/H1 NMR

这项研究的目的是非侵入性地检测体内 ~(13)C核磁研究脑内碳水化合物代谢 共振(核磁共振)。这项工作的目的是开发 人体~(13)C核磁共振波谱仪器的研制 脑生化紊乱的基础研究 精神分裂症和迟发性运动障碍。这些研究将是 在2.1特斯拉和5.0特斯拉下进行，以便S/N比，C-13 T1‘S， 去耦器效率，核Overhauser增强(NOE) 和射频加热可以进行比较。 我们计划在特定情况下利用富含碳-13的葡萄糖 确定体内中间代谢的位置 大脑。C-13标记物的相对量和同一性 新陈代谢中间产物可以通过计算化学物质 C-13或H-1核磁共振谱中每个峰的移位和面积。 我们计划测量葡萄糖碳在 乳酸，以及葡萄糖碳的稀释程度 三元酸循环及其随后的掺入 进入大脑的其他碳库，即谷氨酸、谷氨酰胺， GABA和丙氨酸。 技术和仪器的发展是作为 这项研究应该可以转移到现有的“高场”(2.0T) 二期人体全身临床影像系统 调查。未来的商业化可能会导致 全身或头部系统用仪器的发展 中心磁场大于4.0特斯拉。

The objective of this study is to non-invasively examine in-vivo cerebral carbohydrate metabolism by C-13 nuclear magnetic resonance (NMR). The purpose of this work is to develop instrumentation for C-13 NMR spectroscopy of humans to pursue fundamental research on brain biochemical disorders such as schizophrenia and Tardive Dyskinesia. These studies will be conducted at 2.1 Tesla and 5.0 Tesla so that S/N ratios, C-13 T1's, decoupler efficiency, Nuclear Overhauser Enhancements (NOE) and RF heating may be compared. We plan to utilize glucose, enriched with C-13 at specific positions to determine in-vivo intermediary metabolism of the brain. The relative quantities and identities of C-13 labeled metabolic intermediates can be found by calculating the chemical shift and area for each peak in the C-13 or H-1 NMR spectrum. We plan to measure the incorporation of glucose carbons into lactate, and the extent of dilution of glucose carbons traversing the tricarboxylic acid cycle with their subsequent incorporation into other carbon pools of the brain, i.e. glutamate, glutamine, GABA, and alanine. Techniques and instrumentation developed as a consequence of this research should be transferable to existing "high field" (2.0T) whole body clinical imaging systems for Phase II human investigations. Future commercialization may lead to the development of instrumentation for whole body or head systems with central fields greater than 4.0 Tesla.