CHEMOSENSITIVITY TUMOR ASSAY & INTERPHASE CYTOGENETICS

化学敏感性肿瘤检测

• 批准号: 3493476
• 负责人: HOWARD G GRATZNER
• 金额: $ 4.83万
• 依托单位: ONCYTE, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-30 至 1994-03-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3493476
• 关键词: DNA replication; antineoplastics; bromodeoxyuridine; cell cycle; drug resistance; drug screening /evaluation; in situ hybridization; karyotype; method development; neoplastic growth; prognosis; tissue /cell culture

The objective of this project is to develop a new method for in vitro chemosensitivity and chemoresistance testing of cancer. Phase I will be concerned with an in vitro cell culture system to develop the method, based upon the use of short-term culture of extirpated tumor cells, monitoring the effects of chemotherapeutic drugs on cell proliferation by means of the immunofluorescent detection of bromodeoxyuridine as a measure of DNA synthesis. In order to discriminate tumor cells from the normal diploid stromal cells in the culture, the karyotype of the interphase tumor cells will be will be predetermined by in situ fluorescence hybridation (FISH) prior to BrdUrd pulsing of the cultures, and FISH then performed on the BrdUrd-labeled cells. The dual fluorescence image analysis of IdUrd incorporation and selected FISH probes will distinguish the tumor cells from non-tumor cells in the culture. Corroboration of the identity of tumor cells will be made by a cytopathologist. The method combines the assessment of drug sensitivity of the tumor with the prognostic power of karyotyping.

本项目的目的是开发一种新的体外检测方法， 癌症的化学敏感性和化学抗性测试。 第一阶段将是 涉及体外细胞培养系统以开发该方法， 基于使用摘除的肿瘤细胞的短期培养， 监测化疗药物对细胞增殖的影响， 溴脱氧尿苷的免疫荧光检测方法 DNA合成。 为了区分肿瘤细胞和正常细胞 二倍体基质细胞培养，间期核型 肿瘤细胞将通过原位荧光被预先确定 在培养物的BrdUrd脉冲之前进行杂交（FISH），然后进行FISH， 在BrdUrd标记的细胞上进行。 双荧光图像 IdUrd掺入和所选FISH探针的分析将区分 肿瘤细胞和非肿瘤细胞的区别。 确证 肿瘤细胞的身份将由细胞病理学家确定。 述的方法 将肿瘤的药物敏感性评估与 核型分析的预后能力。