TAXOL FROM SFE EXTRACT BY COUNTERCURRENT CHROMATOGRAPHY

紫杉醇的分离传统上使用三种有机溶剂进行， 溶剂萃取步骤和四个色谱步骤， 太平洋紫杉，短叶红豆杉。 有限的植物资源供应， 低产量刺激了对替代技术的研究， 满足这种实验性抗癌药物的需求。 我们建议 利用一种高效液相色谱法， 超临界流体萃取（SFE） 和离心逆流色谱（CCC）。 这两种技术 可以扩大到加工厂水平。 最近的研究表明 超临界流体萃取是从红豆杉树皮中提取紫杉醇的有效方法 和针头。 富含紫杉醇的提取物可通过CCC分离， 基于液-液分配原理， 柱或HPLC色谱分离。 在第一阶段， 浸提和分离条件将沿着确定， 扩大进程的程序。 CCC的进一步自动化 该装置将简化数据的获取并改进方法 分析和制备工作的验证。

The isolation of taxol is traditionally done using three organic solvent extraction steps and four chromatographic steps from the Pacific yew, Taxus brevifolia. The limited supply of plant sources and low yields have stimulated searches for alternative technologies to meet the demand of this experimental cancer drug. We propose to improve the extraction and isolation of taxol by utilizing a combination of two technologies, supercritical fluid extraction (SFE) and centrifugal countercurrent chromatography (CCC). Both techniques can be scaled up to the processing plant level. Recent work indicates that SFE is an effective method for extracting taxol from Taxus bark and needles. The taxol-rich extract can be separated by CCC, a method based on the liquid-liquid partition principle and is milder than column or HPLC chromatographic separations. In phase I, optimal extraction and isolation conditions will be determined along with the procedures for scaling up the process. Further automation of the CCC apparatus will simplify the acquisition of data and improve method validation for analytical and preparative work.