OPTIMIZING BLOOD COMPONENT HANDLING SYSTEMS

优化血液成分处理系统

• 批准号: 3502370
• 负责人: FRANK H HARMS
• 金额: $ 5万
• 依托单位: LIFESOURCE ADVANCED BLOODBANK SYSTEMS
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-15 至 1994-03-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3502370
• 关键词: blood preservation; clotting factor; cryopreservation; freezing; method development; polyvinyls; temperature

Current technology generally requires over 20 minutes to thaw/warm blood products to transfusion temperature, over 2 hours to completely freeze them, and reduces levels of clotting proteins in plasma. Current plasma bags are susceptible to low temperature fracture and frequently engender plasma/liquid warming medium contamination. Plastic bag over-wraps mitigate contamination dangers but extend the thaw/warming time. These problems cause plasma waste due to thawing in advance of anticipated usage. Preliminary Lifesource data suggest that enhancing freeze/thaw processes by reconfiguring standard blood products bags to optimize the volume/surface area ratio can lead to improved clotting protein levels. These enhancements can be obtained while utilizing a dry thawing medium which eliminates contamination. and is fast enough to allow thawing only for immediate demand. Constructing reconfigured bags from a laminated film appears to alleviate breakage problems. First, we will develop design data for a dry freezing device incorporated into the reconfigured dry thawing device. Second, an appropriately approved film will replace PVC in a reconfigured bag. Third, validate the preliminary results utilizing reconfigured, replacement film, bags. Finally, investigate factors such as time, temperature and processing speed surrounding clotting protein recovery and utilize them to optimize both device and bag performance for commercial development.

目前的技术通常需要超过20分钟来解冻/温暖血液 产品达到输液温度，2小时以上完全冷冻 它们，并降低血浆中凝血蛋白的水平。 电流等离子 袋子容易在低温下破裂， 血浆/液体加温介质污染。 塑料袋外包装 减轻污染危险，但延长解冻/升温时间。 这些 由于在预期时间之前解冻，问题导致血浆浪费 使用. 初步的生命周期数据表明， 通过重新配置标准血液制品袋， 体积/表面积比可以导致改善的凝血蛋白水平。 这些增强可以在使用干燥解冻介质时获得 这消除了污染。并且足够快， 立即需求。 从层压材料构造重新配置的袋 膜似乎减轻了断裂问题。 首先，我们将开发设计数据的干式冷冻装置， 放入重新配置的干燥解冻装置中。 第二，适当 经批准的薄膜将取代重新配置的袋中的PVC。 第三，验证 使用重新配置的替换膜袋的初步结果。 最后，考察时间、温度和加工过程等因素 加速周围凝血蛋白的恢复并利用它们来优化 用于商业开发的器械和袋性能。