CCD CAMERA SYSTEM FOR X-RAY DIFFRACTION IMAGING

用于 X 射线衍射成像的 CCD 相机系统

• 批准号: 3505238
• 负责人: A DANFORTH COPE
• 金额: $ 5万
• 依托单位: PRINCETON SCIENTIFIC INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-18 至 1990-03-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505238
• 关键词: X ray crystallography; fiber optics; image enhancement; image processing; macromolecule; protein structure

This is a proposal to investigate the advantages of a new 2048x2048 pixel CCD type solid state image sensor for X-ray diffraction applications on biological macromolecules. In the proposed experiments the CCD will be fiber optically coupled directly to an X-ray sensitive phosphor screen, eliminating the image intensifier normally employed in such X-ray diffraction imaging equipment. This results in a substantial improvement in spatial resolution and dynamic range. Arrangements have been made to test the CCD imaging system in Prof. Sol Gruner's X-ray diffraction laboratory in Princeton University, Department of Physics. Princeton Scientific Instruments has the 2048x2048 pixel CCD camera system which would be used for these experiments. This grant would support designing and building the fixture for mounting the CCD and fiber optics phosphor screen together and conducting the experiments and analyzing the data. The proposed Phase I program is a cost effective way to investigate the advantages of these new image sensors to X-ray diffraction related research. The high resolution and wide dynamic range of this CCD imaging system is particularly well matched to the problem of X-ray Laue diffraction from protein crystals using synchrotron generated polychromatic X-rays and protein diffraction from large unit cells, a Phase II project.

这是一项调查新的2048x2048像素优势的建议 用于X射线衍射的CCD型固体图像传感器 生物大分子。在拟议的实验中，CCD将被 光纤直接耦合到X射线敏感荧光屏， 消除了这种X射线中通常采用的像增强器 衍射成像设备。这会带来实质性的改善。 在空间分辨率和动态范围上。已作出安排，以 用Sol Gruner教授的X射线衍射仪测试CCD成像系统 普林斯顿大学物理系实验室。普林斯顿 科学仪器公司拥有2048x2048像素的CCD相机系统， 将被用于这些实验。这笔赠款将支持设计 制作了用于安装CCD和光纤荧光粉的夹具 共同筛选并进行实验，并对数据进行分析。 拟议的第一阶段计划是一种具有成本效益的方式，可以调查 这些新型图像传感器在X射线衍射方面的优势 研究。该CCD成像分辨率高、动态范围大 系统特别好地匹配了X射线劳厄的问题 利用同步加速器产生的蛋白质晶体的衍射 大单位细胞的多色X射线和蛋白质衍射，a 二期工程。