MONOCLONAL ANTIBODIES FOR DIRECT STEROID IMMUNOASSAY

第一阶段的目标是建立一种免疫测定法作为模型 并通过高度特异性的单克隆抗体， (MAb)分析准确度上级于目前的商业试剂盒 available. 关键是要获得一个MAb， 特异性和亲和力。 目前可用的免疫测定受到以下问题的困扰： 血清中类固醇浓度的高估，可能是由于 抗体的特异性不足。 A 15羧甲基衍生物 将睾酮与KLH偶联用作抗原， with the requisit要求specificity专一. 选择的单克隆抗体的特异性将 通过测定与其他类固醇的交叉反应性， 血清样本中含有化学确定量的类固醇 在选择高度特异性的单克隆抗体后， 利用荧光剂终点将被开发用于商业用途。 应用程序.

The goal for phase I is to establish, as a model, an immunometric assay for testosterone and achieve, via a highly specific monoclonal antibody, (MAb) an analytical accuracy superior to the commercial kits currently available. The key aspect is to obtain an MAb with the necessary specificity and affinity. Immunoassays currently available suffer from overestimation of the steroid concentration in serum, probably due to an insufficient specificity of the antibody. A 15 carboxymethyl derivation of testosterone coupled to KLH used as an antigen should elicit a MAb with the requisit specificity. The specificity of the MAb selected will be determined assaying crossreactivity with other steroids and testing with serum samples containing chemically determined amounts of steroid. After selection of a highly specific MAb, an immunometric assay utilizing a fluoresant end point will be developed for commercial application.