ELISA KIT SPECIFIC FOR THE ANTI-PHOSPHOLIPID SYNDROME

在一些患者中，磷脂结合抗体与 复发的静脉和/或动脉血栓形成以及复发的胎儿 丧失(“抗磷脂综合征”(APS))。这些抗体是由 在各种临床情况下，上述情况很可能 临床联系仅限于患有特殊疾病的患者 抗磷脂(APL)抗体亚群。抗体亚群可以 在它们对特定磷脂的亲和力或在它们的 特定磷脂构象的特异性，这种构象 由磷脂-脂类混合物测定。有可能是 磷脂抗原浓度或磷脂脂类的操纵 酶联免疫吸附试验检测系统中的混合物可以实现更特异的鉴定 APS患者的比例。 以期制备一种可销售的试剂盒，该试剂盒专门设计用于 鉴别APS患者，本研究建议与ELISA法进行比较 具有不同浓度的负电荷磷脂和 磷脂-脂质混合物，以确定哪一种体系最 对鉴别APS患者具有敏感性和特异性。中使用的材料 检测系统将随着时间的推移对其稳定性进行评估，以确定 它们在酶联免疫吸附试验试剂盒中的应用。

In some patients, phospholipid binding antibodies have been associated with recurrent venous and/or arterial thrombosis as well as with recurrent fetal loss (the "Antiphospholipid Syndrome" (APS)). These antibodies are induced in a variety of clinical circumstances and it is likely that the above clinical associations are limited only to patients with particular subgroups of anti-phospholipid (aPL) antibodies. Antibody subgroups may differ in their affinities for particular phospholipids or in their specificities for particular phospholipid conformations, such conformations being determined by phospholipid-lipid mixtures. It is possible that manipulation of phospholipid antigen concentration or of phospholipid-lipid mixtures in an ELISA assay system may enable more specific identification of patients with the APS. With a view to preparing a marketable ELISA kit specifically designed to identify patients with the APS, this study proposes to compare ELISA assay systems with varying concentrations of negatively charged phospholipids and phospholipid-lipid mixtures, to determine the system which is most sensitive and specific for identifying APS patients. Materials used in the assay systems will be evaluated for their stability over time to determine their utility in an ELISA assay kit.