DETECTION OF THEOPHYLLINE USING A SIMPLE IMMUNOASSAY

这项工作的长期目标是开发一种快速，比色， 茶碱成分仪器独立测定。 Genesis Labs的 “IMMUNOSTRIP”测定法（受专利保护）构建在 配置，适用于药物的快速检测， 生物流体中的代谢物，例如尿液、血液或唾液。 的 该方法的相对简单性表明其易于在医学上使用， 单纯的人 我们将首先尝试确定 血清中茶碱水平为5 ng/ml。 最终，我们的目标是 将测定系统的灵敏度提高到1-5 ng/ml范围。 这将需要使用手持式反射计（每台1000美元 用于阅读条带;然而，通过改变 抗原和酶修饰的抗茶碱的量 IMMUNOSTRIP，我们可以在此较低范围内进行目视检测。 该测定具有许多用于监测治疗药物的应用，包括 茶碱，因此将对我们的知识产生重大影响， 健康利益/风险比在我们的社会人口谁需要 毒品 基于我们监测其他激素、药物和小 半抗原，我们相信，在未来，通过使用 高度特异性的多克隆抗血清或更可能的单克隆抗体， 可以监测活性茶碱和非活性代谢物， 从而预测治疗水平与毒性水平 茶碱在医生的办公室设置与一分钟的测试。

The long term objectives of this work is to develop a rapid, colorimetric, instrument independent assay for theophylline constituents. Genesis Labs' "IMMUNOSTRIP" assay (protected by patent) is constructed in a configuration, which is applicable for the rapid detection of drugs and metabolites in biological fluids, e.g. urine, blood or saliva. The relative simplicity of the method suggests its easy use by medically unsophisticated individuals. We will attempt, initially, to determine theophylline in serum at a level of 5 ng/ml. Ultimately, our aim is to increase the sensitivity of the assay system into the 1-5 ng/ml range. This will require the use of a hand held reflectometer ($1000 per unit currently) for reading the strips; however, it is feasible by varying the amount of antigen and enzyme modified antitheophylline within the IMMUNOSTRIP that we can produce a visual assay within this lower range. The assay has many applications for monitoring therapeutic drugs, including theophylline, and therfore will have a major impact on our knowledge of the health benefit/risk ratio within populations of our society who require drugs. Based on our ability to monitor other hormones, drugs and small haptens using this assay, we feel confident that in the future by using highly specific polyclonal antisera or more likely monoclonal antibodies, it may be possible to monitor active theophylline and inactive metabolites in blood and thereby predict therapeutic versus toxic levels of theophylline in a physician's office setting with a one minute test.