ANALYSIS OF DIGOXIN IN USING A SIMPLE IMMUNOASSAY

这项工作的长期目标是开发一种快速、比色的 值得注意的是，心脏洋地黄成分的非仪器测定 地高辛和洋地黄毒素。Genesis Labs的“IMMNOSTRIP”检测(受保护 专利)被构造为一种适用于 快速检测生物体液中的药物和代谢物，例如尿液， 血或唾液。这种方法相对简单，说明它很容易。 由医学上不成熟的个人使用。我们最初会尝试， 测定血清中地高辛的浓度为5 ng/ml。最终，我们的目标是 是将检测系统的灵敏度提高到1-5 ng/ml 射程。这将需要使用手持反射仪(每个1000美元 当前单元)用于读取条带，然而，通过改变 抗原和酶修饰的抗地高辛抗体在体内的量 我相信我们可以在这个较低的范围内进行目视分析。 该分析在监测治疗药物方面有许多应用，包括 ，因此将对我们对地高辛的认识产生重大影响 我们社会中需要以下需求的人群中的健康收益/风险比率 毒品。基于我们监测其他荷尔蒙、药物和小分子 使用这种分析方法，我们有信心在未来通过使用 高度特异的多克隆抗血清或更有可能的单抗， 有可能监测活性地高辛并由此预测 医生办公室环境中地高辛的治疗水平与毒性水平 只有一分钟的测试。

The long term objectives of this work is to develop a rapid, colorimetric instrument independent assay for cardio digitalis constituents, notably digoxin and digitoxin. Genesis Labs' "IMMUNOSTRIP" assay (protected by patent) is constructed in a configuration, which is applicable for the rapid detection of drugs and metabolites in biological fluids e.g. urine, blood or saliva. The relative simplicity of the method suggests its easy use by medically unsophisticated individuals. We will attempt initially, to determine digoxin in serum at a level of 5 ng/ml. Ultimately, our aim is to increase the sensitivity of the assay system into the 1-5 ng/ml range. This will require the use of a hand held reflectometer ($1000 per unit currently) for reading the strips, however it is feasible by varying the amount of antigen and enzyme modified anti-digoxin antibody within the IMMUNOSTRIP that we can produce a visual assay within this lower range. The assay has many applications for monitoring therapeutic drug including digoxin, and therefore will have a major impact on our knowledge of the health benefit/risk ratio within populations of our society who require drugs. Based on our ability to monitor other hormones, drugs and small haptens using this assay, we feel confident that in the future by using highly specific polyclonal antisera or more likely monoclonal antibodies, it may be possible to monitor active digoxin and thereby predict therapeutic versus toxic levels of digoxin in a physician's office setting with a one minute test.