BLOOD CELL TYPING USING OPTICAL PHASE AND FLOW CYTOMETRY

鉴于目前的压力， 临床成本控制。由于血液分类和筛查是一项 众所周知，临床实验室是劳动密集型领域， 制造商集中精力开发各种ABO/Rh血型 技术. 我们很少有人认识到在表演中需要专业化 这些测试，以及这些测试对血液的轻微影响， 输血实验室。 另一方面，免疫学的新技术 和血液学，如灵敏和客观的"高端"流式细胞术 已经取代了视觉观察，但仍然过于复杂和昂贵， 血液银行家使用。 然而，还没有工具可用于执行这些类型 测量方法的任何不同，更好或更便宜。 在这个建议中，我们描述了一种方法，既适合血库 免疫血液学实验室 这项技术有可能将联合收割机 在简单的流程中进行红细胞分型和淋巴细胞亚群分析 流式细胞仪 这种方法可以克服以下问题： 背景荧光干扰 这是使用光学相位 用双频HeNe激光测量颗粒干质量 干涉配置中的源。 测量灵敏度 通过使用对细胞膜特异的微珠来增强。

Labor saving techniques are essential given the current pressure on clinical cost containment. Since blood classification and screening is a notoriously labor intensive area of the clinical laboratory, manufacturers have concentrated on developing various ABO/Rh typing techniques. Few of us recognize the specialization needed in performing such tests, and the minor impact these tests have had in the blood transfusion laboratory. On the other hand, new techniques in immunology and hematology such as sensitive and objective "high-end" flow cytometry has replaced visual observation but remains too complex and costly for use by blood bankers. Yet, there is no tool available to do these types of measurements any differently, better or less expensively. In this proposal we describe a method suitable for both the blood bank and immunohematology laboratory. The technique can potentially combine red blood cell typing and lymphocyte subset analysis in a simple flow cytometry instrument. This approach can overcome the problem of background fluorescence interference. This is done using optical phase measurements of particle dry mass using a two-frequency HeNe laser source in an interferometric configuration. Measurement sensitivity is enhanced by the use of microbeads specific to cell membranes.