NEW FLOW CYTOMETRY TECHNOLOGIES FOR CLINICAL HEMATOLOGY

用于临床血液学的新型流式细胞术技术

• 批准号: 3501873
• 负责人: DOUGLAS E BURGER
• 金额: $ 4.86万
• 依托单位: ABIOMED, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3501873
• 关键词: biomedical equipment resource; blood tests; cell morphology; clinical biomedical equipment; erythrocyte count; flow cytometry; fluorescence; hemoglobin; leukocyte count

Automated blood cell analysis is a routine function in the clinical hematology laboratory. We have all had simples taken for such a purpose, but very few realize that the flow cytometric instrumentation performing these tests may have fundamental limitations regarding measurement of red blood cell volume, red blood cell hemoglobin concentration, and accurate classification of white blood cell subpopulations. The `high-end' instruments have become very complex and expensive in order to `ensure' that an accurate and reliable result can be reported. Yet, there is no tool available to do these kinds of measurements any differently or better. In this proposal is described two methods for the clinical flow cytometry hematology laboratory that can overcome the problem associated with hemoglobin concentration and volume interferences when making red blood cell measurements and overcome the problem of refractive index and volume interference in the white blood cell differential measurement. This is done by simultaneously detearmining cell dry mass by measurements of phase using a two-frequency HeNe laser and cell volume by the principle of dye exclusion. This Phase I, research will characterize both techniques using particle and blood samples, on an experimental flow cytometer. The ultimate instrument will provide a unique capability as a low cost device for making accurate red blood cell and white blood cell dry mass and volume measurements.

自动血细胞分析是临床中的常规功能， 血液学实验室 我们都有过类似的经历， 但很少有人意识到， 这些测试对于红色的测量可能具有基本的局限性 血细胞体积、红细胞血红蛋白浓度， 白色血细胞亚群的分类。 “高端” 工具变得非常复杂和昂贵，以“确保” 可以报告准确和可靠的结果。 然而，没有 工具可用于做这些类型的测量任何不同或更好。 在这个建议中，描述了两种用于临床流式细胞术的方法 血液学实验室，可以克服与 制备红血时血红蛋白浓度和体积干扰 细胞测量和克服的问题，折射率和体积 干扰白色血细胞分类测定。 这是 通过测量相位同时测定细胞干质量来完成 利用双频HeNe激光器和细胞容积染料原理 排斥。 第一阶段的研究将描述这两种技术使用粒子和 血液样本，在实验性流式细胞仪上。 终极工具 将提供作为低成本装置的独特能力， 红细胞和白色细胞干质量和体积测量。