ACOUSTO-OPTIC DEVICES FOR TIME-RESOLVED FLUORESCENCE

用于时间分辨荧光的声光装置

• 批准号: 3498634
• 负责人: RONALD G ROSEMEIER
• 金额: $ 5万
• 依托单位: BRIMROSE CORPORATION OF AMERICA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498634
• 关键词: biomedical equipment development; chemical structure; fluorescence; fluorimetry; lasers; luminescence; photobiology; time resolved data

DESCRIPTION (Adapted from applicant's abstract): Numerous areas of biochemical, biophysical and medical research depend upon measurements of time-resolved fluorescence and more recently, of time-dependent photon migration in tissues. In recent years these phenomenan have been studied by the technique of frequency-domain fluorometry (FREDOF), which provides good signal-to-noise, ease of use, and good resolution of complex decays. However, the time-resolution of FREDOF is currently limited by the use of electro-optic (E-O) devices which allow light modulation to only 200 MHz with approximately 5 percent transmission. The long range goal of this program is to circumvent these frequency and transmission limitations by the use of advanced acousto-optic technology of which Brimrose is a high frequency MHz-GHz Acousto-Optic manufacturer. As a Phase I goal it is proposed to fabricate the acousto-optic modulators with a light-modulation bandwidth of DC to 200 MHz, with at least 50 percent of the light in the modulated beam. Additionally, these devices will provide transmission from 200-1000nm, so as to allow its use in diverse studies ranging from intrinsic DNA and protein luminescence (250-300nm) to studies of NIR dyes for clinical sensing (600-800nm). The devices will be designed for use with laser sources, such as the CW argon ion laser (275nm). These devices will be tested and evaluated at the University of Maryland Medical School, Center for Fluorescence Spectroscopy.

描述(改编自申请人的摘要)：多个领域 生化、生物物理和医学研究依赖于对 时间分辨荧光，以及最近的依赖于时间的光子 在组织中的迁移。近年来，人们对这些现象进行了研究 通过频域荧光测量(FREDOF)技术，提供了 良好的信噪比、易用性和对复杂衰减的良好分辨率。 然而，FREDOF的时间分辨率目前受到使用的限制 仅允许光调制到200 MHz的电光(E-O)设备 大约5%的传播率。 该计划长期目标是绕过这些频率和 使用先进的声光技术的传输限制 布里姆罗斯是一家高频MHz-GHz声光制造商。AS 第一阶段的目标是制作声光调制器。 光调制带宽DC至200 MHz，至少50% 调制光束中的光的百分比。此外，这些设备 将提供200-1000 nm的传输，以便将其用于 从本征DNA和蛋白质发光的不同研究 (250-300 nm)用于临床传感用近红外染料的研究(600-800 nm)。这个 设备将设计为与激光光源一起使用，如CW Ar 离子激光(275 Nm)。 这些设备将在马里兰大学进行测试和评估 医学院，荧光光谱学中心。