ACOUSTO-OPTIC DEVICES FOR TIME-RESOLVED FLUORESCENCE

描述（改编自申请人的摘要）： 生物化学、生物物理学和医学研究依赖于 时间分辨荧光和最近的时间相关光子 组织中的迁移。 近年来，这些现象已被研究 通过频域荧光测定法（FREDOF）技术， 良好的信噪比、易用性和复杂衰减的良好分辨率。 然而，FREDOF的时间分辨率目前受到使用 电光（E-O）器件，其允许光调制到仅200 MHz 大约5%的传输率。 该计划的长期目标是规避这些频率， 先进的声光技术的使用， Brimrose是一家高频MHz-GHz声光器件制造商。 作为 第一阶段的目标是制造声光调制器， 具有DC至200 MHz的光调制带宽，具有至少50 调制光束中的光的百分比。 此外，这些设备 将提供200- 1000 nm的传输，以便允许其用于 从内在DNA和蛋白质发光 （250- 300 nm）的近红外染料的研究用于临床传感（600- 800 nm）。 的 这些装置将被设计成与激光源一起使用，例如连续波氩激光器 离子激光（275 nm）。 这些器械将在马里兰州大学进行测试和评估 医学院，荧光光谱中心。

DESCRIPTION (Adapted from applicant's abstract): Numerous areas of biochemical, biophysical and medical research depend upon measurements of time-resolved fluorescence and more recently, of time-dependent photon migration in tissues. In recent years these phenomenan have been studied by the technique of frequency-domain fluorometry (FREDOF), which provides good signal-to-noise, ease of use, and good resolution of complex decays. However, the time-resolution of FREDOF is currently limited by the use of electro-optic (E-O) devices which allow light modulation to only 200 MHz with approximately 5 percent transmission. The long range goal of this program is to circumvent these frequency and transmission limitations by the use of advanced acousto-optic technology of which Brimrose is a high frequency MHz-GHz Acousto-Optic manufacturer. As a Phase I goal it is proposed to fabricate the acousto-optic modulators with a light-modulation bandwidth of DC to 200 MHz, with at least 50 percent of the light in the modulated beam. Additionally, these devices will provide transmission from 200-1000nm, so as to allow its use in diverse studies ranging from intrinsic DNA and protein luminescence (250-300nm) to studies of NIR dyes for clinical sensing (600-800nm). The devices will be designed for use with laser sources, such as the CW argon ion laser (275nm). These devices will be tested and evaluated at the University of Maryland Medical School, Center for Fluorescence Spectroscopy.