DEVELOPMENT OF AN AUTOMATED SEGMENTED DNA SYNTHESIZER

自动分段 DNA 合成仪的开发

• 批准号: 3500480
• 负责人: JAMES D FROST
• 金额: $ 5万
• 依托单位: SIGMA-GENOSYS, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3500480
• 关键词: DNA; biomedical automation; biomedical equipment development; nucleic acid chemical synthesis; oligonucleotides; polynucleotides; solvents

This proposal relates to the development of an automated DNA synthesizer that is orders of magnitude faster and more cost effective than current commercial DNA synthesizers while utilizing standard DNA chemistry. The goal of this research proposal is to automate the solvent delivery system for the Wafer DNA synthesis device and to eventually automate all aspects of the device. Once complete automation is achieved, the Wafer DNA synthesis device is expected to reduce the reagent cost per base addition from the current two to five dollars (o.2 mumol scale of synthesis by conventional DNA synthesizers) to under fifty cents. The performance capability of one automated machine with its faster production time and lower cost of production should dramatically reduce the cost and time associated with sequencing projects, gene synthesis, protein engineering, DNA amplification, probe optimization and other genetic engineering research. With the need for hundreds of thousands to millions of polynucleotides for the Human Genome project alone, we believe the development of the semi-automated Wafer DNA synthesizer into a fully automated machine will have a major impact in the progress of DNA related research.

这项提议涉及到自动化DNA的发展 速度更快、成本效益更高的合成器 比目前的商用DNA合成器更好，同时使用标准DNA 化学反应。这项研究提案的目标是将 用于晶片DNA合成装置和TO的溶剂输送系统 最终实现设备所有方面的自动化。一旦完成 实现自动化，晶片DNA合成装置有望 将每个碱基添加的试剂成本从目前的2个减少到5个 美元(0.2摩尔规模的常规DNA合成 合成器)降到50美分以下。一个人的表演能力 以其更快的生产时间和更低的成本 生产应该大大减少相关的成本和时间 测序项目，基因合成，蛋白质工程，DNA 扩增、探针优化等基因工程 研究。需要数十万到数百万的 仅用于人类基因组计划的多核苷酸，我们相信 半自动晶片DNA合成仪向全自动化方向的发展 自动化机器将对DNA的进步产生重大影响 相关研究。