DEVELOPMENT OF AN AUTOMATED SEGMENTED DNA SYNTHESIZER

自动分段 DNA 合成仪的开发

• 批准号: 3508327
• 负责人: JAMES D FROST
• 金额: $ 21万
• 依托单位: SIGMA-GENOSYS, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508327
• 关键词: DNA; biomedical automation; biomedical equipment development; computer program /software; nucleic acid chemical synthesis; oligonucleotides; phosphorus compounds

DESCRIPTION (Adapted from applicant's abstract):This proposal relates to the automated simultaneous chemical synthesis of large numbers of high quality oligonucleotides at a total throughput greater than four bases per minute using beta-cyanoethyl phosphoramidite chemistry, and reagent usage at a fraction of conventional automated DNA synthesizers. One goal of this Phase II research is to fabricate a fully automated, multiple DNA synthesizer capable of producing 40 - 60 oligonucleotides simultaneously at 0.02 - 0.2 umol scale, reducing the reagent cost per base addition from the current $1.00 to $2.00 (0.2 umol scale of synthesis by conventional DNA synthesizers) to under 50 cents.The performance capability of the automated machine with its faster production time and lower cost of production should dramatically reduce the cost and time associated with research projects requiring numerous oligonucleotides. Besides fabricating a fully automated system which could be produced at a cost affordable to researchers, another Phase II objective is to produce a higher throughput, semiautomated instrument for the HGP, for example oligonucleotide libraries, STS primer, repository and oligonucleotide arrays. The Phase II research is expected to stimulate further technology development in the HGP.

描述（改编自申请人的摘要）：该提案涉及 大量高活性物质的自动同步化学合成 高质量寡核苷酸的总通量大于每个碱基四个 分钟使用 β-氰乙基亚磷酰胺化学和试剂使用 仅需传统自动化 DNA 合成仪的一小部分。这一目标之一 第二阶段研究是制造全自动、多重 DNA 能够同时生产 40 - 60 个寡核苷酸的合成仪 0.02 - 0.2 umol 规模，降低每次添加碱基的试剂成本 目前 1.00 美元至 2.00 美元（传统 DNA 合成的 0.2 umol 规模） 合成器）低于 50 美分。自动化的性能 机器具有更快的生产时间和更低的生产成本 大大减少与研究项目相关的成本和时间 需要大量的寡核苷酸。 除了制造一个可以在 研究人员可以承受的成本，第二阶段的另一个目标是生产 例如，用于 HGP 的更高通量的半自动化仪器 寡核苷酸文库、STS 引物、存储库和寡核苷酸 数组。 二期研究预计将刺激进一步的技术发展 人类基因组计划的发展。