DEVELOPMENT OF AN AUTOMATED SEGMENTED DNA SYNTHESIZER

描述(改编自申请人的摘要)：本建议书涉及 大量高纯度化合物的自动同时化学合成 总产量超过每个碱基4个碱基的优质寡核苷酸 使用β-氰乙基亚磷酰胺化学和试剂使用 只有传统自动DNA合成器的一小部分。其中一个目标就是 第二阶段的研究是制造全自动的、多个DNA 一种可同时生产40-60个寡核苷酸的合成器 0.02-0.2微克分子刻度，降低了每个碱基添加的试剂成本 目前1.00美元至2.00美元(0.2微摩尔的传统DNA合成规模 合成器)到50%以下。自动化的性能 生产时间更快、生产成本更低的机器应该 大幅降低与研究项目相关的成本和时间 需要大量的寡核苷酸。 除了制造一个可以在一年内生产的全自动系统 研究人员负担得起的成本，第二阶段的目标是生产一种 更高的吞吐量，例如用于HGP的半自动仪器 寡核苷酸文库、STS引物、储存库和寡核苷酸 数组。第二阶段的研究预计将刺激进一步的技术 人类基因组计划的发展。

DESCRIPTION (Adapted from applicant's abstract):This proposal relates to the automated simultaneous chemical synthesis of large numbers of high quality oligonucleotides at a total throughput greater than four bases per minute using beta-cyanoethyl phosphoramidite chemistry, and reagent usage at a fraction of conventional automated DNA synthesizers. One goal of this Phase II research is to fabricate a fully automated, multiple DNA synthesizer capable of producing 40 - 60 oligonucleotides simultaneously at 0.02 - 0.2 umol scale, reducing the reagent cost per base addition from the current $1.00 to $2.00 (0.2 umol scale of synthesis by conventional DNA synthesizers) to under 50 cents.The performance capability of the automated machine with its faster production time and lower cost of production should dramatically reduce the cost and time associated with research projects requiring numerous oligonucleotides. Besides fabricating a fully automated system which could be produced at a cost affordable to researchers, another Phase II objective is to produce a higher throughput, semiautomated instrument for the HGP, for example oligonucleotide libraries, STS primer, repository and oligonucleotide arrays. The Phase II research is expected to stimulate further technology development in the HGP.