AN IMPROVED REPLACEMENT TECHNOLOGY FOR RFLP ANALYSIS

一种改进的 RFLP 分析替换技术

• 批准号: 3507921
• 负责人: DONNA M SHATTUCK-EIDENS
• 金额: $ 18.44万
• 依托单位: AGRIDYNE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-15 至 1992-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507921
• 关键词: animal breeding; biomedical equipment development; genetic mapping; genetic markers; genome; plant genetics; polymerase chain reaction; restriction fragment length polymorphism; southern blotting

RFLP analysis has been shown to have tremendous implications for both the diagnosis of genetic tendencies for human disease and for applications to plant the capacity of what is primarily a research technology. A replacement technology is desired that is faster, cheaper, and with a higher throughput but yet at the same time yields comparable information. NPI has developed a PCR-based approach which appears to satisfy many of these criteria. This process delivers information as to the DNA sequence variability between individuals at specific loci which can then be used analogously to RFLP results. Reduction to practice requires that a better understanding of amplification kinetics be developed, exact conditions for sequence discrimination be further refined, and implementation of a capture-signal strategy is essential to remove the necessity for radioactive labels and gel analysis. The resulting process should require less than a day for completion, be cheaper by ten-fold over conventional less analysis, and facilitate the analysis of several loci for many samples, all in a laboratory with personnel much less sophisticated than now required.

RFLP分析已经被证明对两个方面都有巨大的影响 人类疾病遗传倾向的诊断及其在临床上的应用 种植主要是一项研究技术的能力。一个 需要速度更快、成本更低且具有 更高的吞吐量，但同时产生了可比的信息。 NPI已经开发了一种基于PCR的方法，似乎满足了许多 这些标准。这个过程传递有关DNA序列的信息 特定基因座上的个体之间的变异性，然后可以利用 与RFLP结果类似。简化为实践需要更好的 对扩增动力学的理解，准确的条件 进一步细化序列识别，并实现 捕获信号策略是消除以下必要性的关键 放射性标签和凝胶分析。由此产生的过程应该需要 不到一天的完工时间，比传统的便宜十倍 更少的分析，并促进对多个基因座的分析 样品，都在实验室里，人员远不如 现在是必需的。

项目成果

Rapid detection of maize DNA sequence variation.

快速检测玉米DNA序列变异。

• DOI: 10.1016/1050-3862(91)90035-p
• 发表时间: 1991
• 期刊: Genetic analysis, techniques and applications
• 作者: Shattuck-Eidens,DM;Bell,RN;Mitchell,JT;McWhorter,VC
• 通讯作者: McWhorter,VC