AN IMPROVED REPLACEMENT TECHNOLOGY FOR RFLP ANALYSIS

一种改进的 RFLP 分析替换技术

• 批准号: 3507920
• 负责人: DONNA M SHATTUCK-EIDENS
• 金额: $ 20.17万
• 依托单位: AGRIDYNE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-15 至 1992-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507920
• 关键词: DNA; animal breeding; biomedical equipment development; genetic mapping; genetic markers; genome; plant genetics; polymerase chain reaction; restriction fragment length polymorphism

RFLP analysis has been shown to have tremendous implications for both the diagnosis of genetic tendencies for human disease and for applications to plant the capacity of what is primarily a research technology. A replacement technology is desired that is faster, cheaper, and with a higher throughput but yet at the same time yields comparable information. NPI has developed a PCR-based approach which appears to satisfy many of these criteria. This process delivers information as to the DNA sequence variability between individuals at specific loci which can then be used analogously to RFLP results. Reduction to practice requires that a better understanding of amplification kinetics be developed, exact conditions for sequence discrimination be further refined, and implementation of a capture-signal strategy is essential to remove the necessity for radioactive labels and gel analysis. The resulting process should require less than a day for completion, be cheaper by ten-fold over conventional less analysis, and facilitate the analysis of several loci for many samples, all in a laboratory with personnel much less sophisticated than now required.

RFLP分析已被证明对两个方面都有巨大的影响。 人类疾病遗传倾向的诊断及其在 植物的能力主要是一种研究技术。 一 需要更快、更便宜并且具有更高的成本的替代技术。 更高的吞吐量，但同时产生可比较的信息。 NPI开发了一种基于PCR的方法，该方法似乎满足了许多 这些标准。 这一过程传递的信息， 个体之间在特定基因座的变异性， 类似于RFLP结果。 减少实践需要更好的 发展对扩增动力学的理解， 序列判别进一步完善，并实施一个 捕获信号策略对于消除 放射性标记和凝胶分析。 由此产生的过程应要求 不到一天的时间完成，比传统的便宜十倍 分析少，便于多个位点分析 样品，所有这些都在一个实验室里， 现在需要。