ADAPT ELISA/IFAT FOR SERODIAGNOSIS OF TYZZER'S

采用 ELISA/IFAT 进行 TYZZER 血清诊断

• 批准号: 3505190
• 负责人: ROBERT L PETERS
• 金额: $ 5万
• 依托单位: MICROBIOLOGICAL ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1988-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505190
• 关键词: bacterial disease; enzyme linked immunosorbent assay; gram negative bacteria; immunofluorescence technique; serology /serodiagnosis

Microbiolgoically-defined laboratory animals are required for sophisticated biomedical research. Serological procedures are the most feasible and economical means to monitor their health status. Tyzzer's disease is one of the more insidious diseases of laboratory rodents and rabbits for which a serologic test procedure is not available commercially. The elusive fastidious etiologic agent, Bacillus piliformis (N.SP., Tyzzer), parasite which remains unclassified. Typical histopathology forms the basis for diagnosis in 18 animal species. Immunosuppression precipitates clinical disease. Transmission occurs vertically as well as horizontally. Serological surveys in Japan and Europe indicate that inapparent infections are common in cesarean-derived, barrier-maintained colonies of rats. Microbiological Associates, Inc. proposes to prepare seed pools of 5 strains of B. piliformis (propagated in embryonated eggs and primary hepatocyte cell cultures), prepare antisera to each strain in mice and rats, adapt the ELISA and IFAT techniques for serodiagnosis, determine the degree of antigenic relatedness between strains, and provide serodiagnostic services to the biomedical research community at reasonable cost.

需要微生物定义的实验室动物， 尖端的生物医学研究 血清学程序是 最可行和最经济的方法来监测他们的健康 status. 泰泽氏病是一种比较隐蔽的疾病， 实验室啮齿动物和兔子， 该方法在商业上不可用。 难以捉摸的挑剔 病原体，发状芽孢杆菌（N.SP.，Tyzzer），寄生虫， 仍然未分类。 典型的组织病理学形成了 18种动物的诊断。 免疫抑制沉淀物 临床疾病 垂直传播， 水平。 日本和欧洲的血清学调查表明， 隐性感染在剖腹产中很常见， 屏障维持的大鼠群体。 微生物协会， Inc.建议制备5株B的种子库。毛状的 （在含胚卵和原代肝细胞中繁殖） 培养物），制备小鼠和大鼠中每种菌株的抗血清， 应用ELISA和IFAT技术进行血清学诊断， 菌株之间的抗原相关性程度，并提供 为生物医学研究界提供血清诊断服务. 合理的成本。