PRODUCTION OF MURINE DIAGNOSTIC REFERENCE REAGENTS

鼠诊断参考试剂的生产

• 批准号: 3505993
• 负责人: ROBERT L PETERS
• 金额: $ 22.11万
• 依托单位: MICROBIOLOGICAL ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505993
• 关键词: Mycoplasma; animal care; antibacterial antibody; antigens; antiserum; antiviral antibody; bacterial disease; communicable disease diagnosis; complement fixation tests; diagnosis quality /standard; enzyme linked immunosorbent assay; immunization; immunofluorescence technique; immunologic assay /test; immunological substance; laboratory mouse; laboratory rat; reagent /indicator; serology /serodiagnosis; serotyping; veterinary medicine; virus classification; virus diseases

Microbiologically-defined mice and rats are required for sophisticated biomedical research. Serological procedures are the most feasible and economical means to monitor the health status of laboratory animals. A major problem arises when the results of various "test-kits" and in-house testing laboratories (government, industry and academia) are compared. The lack of standardization of test procedures and the unavailability of reference reagents (quality control) results in poor comparability and engenders a lack of confidence in laboratory animal health monitoring programs designed to safeguard the integrity of large studies involving very large sums of money. Microbiological Associates lnc. proposes to produce monospecific antisera to twenty-five (25) murine infectious agents (in addition to the 4 agents under Phase I) to be used as reference reagents in the standardization of serodiagnostic procedures (CF, HAl, ELlSA and IFA). A key feature of this proposal is the oversight, analysis and recommendations during all phases of the project by an independent Scientific Advisory Committee composed of experts in the fields of microbiology, immunology and laboratory animal medicine. The findings and recommendations of the committee will be publicized.

微生物学定义的小鼠和大鼠需要 先进的生物医学研究。血清学程序是 监测健康状况的最可行和最经济的手段 实验动物。当结果出现时，一个大问题就出现了 各种“测试工具包”和内部测试实验室 (政府、行业和学术界)进行了比较。缺乏 测试程序的标准化和无法获得 参比试剂(质控)可比性差 并导致对实验动物健康缺乏信心 旨在保护大型数据库完整性的监控计划 涉及大量资金的研究。微生物学 联营公司。建议生产单特异性抗血清以 二十五(25)种小鼠感染剂(除了4种 第I阶段中的试剂)将用作 血清诊断程序标准化(CF、Hal、ELLSA和 IFA)。这项建议的一个关键特点是监督、分析 并在项目的所有阶段由 由下列专家组成的独立科学咨询委员会 微生物学、免疫学和实验动物领域 医药。委员会的调查结果和建议将 公之于众。