PRODUCTION OF MURINE DIAGNOSTIC REFERENCE REAGENTS

鼠诊断参考试剂的生产

• 批准号: 3505994
• 负责人: ROBERT L PETERS
• 金额: $ 20.5万
• 依托单位: MICROBIOLOGICAL ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505994
• 关键词: Mycoplasma; animal care; antibacterial antibody; antigens; antiserum; antiviral antibody; bacterial disease; communicable disease diagnosis; complement fixation tests; diagnosis quality /standard; enzyme linked immunosorbent assay; immunization; immunofluorescence technique; immunologic assay /test; immunological substance; laboratory mouse; laboratory rat; reagent /indicator; serology /serodiagnosis; serotyping; veterinary medicine; virus classification; virus diseases

Microbiologically-defined mice and rats are required for sophisticated biomedical research. Serological procedures are the most feasible and economical means to monitor the health status of laboratory animals. A major problem arises when the results of various "test-kits" and in-house testing laboratories (government, industry and academia) are compared. The lack of standardization of test procedures and the unavailability of reference reagents (quality control) results in poor comparability and engenders a lack of confidence in laboratory animal health monitoring programs designed to safeguard the integrity of large studies involving very large sums of money. Microbiological Associates lnc. proposes to produce monospecific antisera to twenty-five (25) murine infectious agents (in addition to the 4 agents under Phase I) to be used as reference reagents in the standardization of serodiagnostic procedures (CF, HAl, ELlSA and IFA). A key feature of this proposal is the oversight, analysis and recommendations during all phases of the project by an independent Scientific Advisory Committee composed of experts in the fields of microbiology, immunology and laboratory animal medicine. The findings and recommendations of the committee will be publicized.

需要微生物定义的小鼠和大鼠 尖端的生物医学研究 血清学程序是 最可行和最经济的方法来监测健康状况 实验室动物 一个主要的问题是， 各种"测试包"和内部测试实验室 （政府、工业界和学术界）进行比较。 缺乏 测试程序的标准化和 参比试剂（质控品）可比性差 并导致对实验室动物健康缺乏信心 旨在保护大型企业的完整性的监测计划 涉及巨额资金的研究。 微生物 联合公司建议生产单特异性抗血清， 二十五（25）种鼠传染性病原体（除4种 I期试剂）用作参比试剂 标准化血清学诊断程序（CF、HA1、ELISA和 IFA）。 该提案的一个主要特点是监督、分析 在项目的所有阶段， 独立的科学咨询委员会， 微生物学、免疫学和实验动物领域 药 委员会的调查结果和建议将 被宣传。