LIPOPHILIC LI+ ION FLUOROPHORES

我们建议合成各种亲油的锂离子荧光团。 最终将在细胞感兴趣的应用中找到用途 生理学家、神经科科学家和治疗病人的精神病学家 表现出躁狂/抑郁精神错乱。在第一阶段，我们已经准备好 这些荧光团中的几个。其中一个的Li/Na高5倍 选择性，基于竞争性提取评估，比任何 文献中报道了亲脂性锂离子载体。这种化合物 对锂离子的荧光也有显著的增强。 在第二阶段，我们希望合成更多这样的化合物，这些化合物是 可能会在第一阶段工作的基础上改进选择性和 荧光特性。我们还将探索这些化合物在 设计一种简易的现场试验来测定锂的浓度 一滴血。这样的测试不需要技术人员绘制 血液样本可以很容易地在精神科医生的办公室进行 确定患者对碳酸锂治疗的依从性。其他 离子选择电极(ISE)等化合物的应用 细胞用水溶性类似物的制备与合成 锂离子对肌醇抑制作用的生理学研究 磷酸盐代谢和其他细胞机制也将被探索。

We propose to synthesize a variety of lipophilic lithium ion fluorophores which will ultimately find use in applications of interest to cell physiologists, neuro scientists and to psychiatrists who treat patients manifesting manic/depressive psychosis. In Phase I, we have prepared several of these fluorophores. One of them has 5-fold greater Li/Na selectivity, based on a competitive extraction evaluation, than any lipophilic lithium ion carrier reported in the literature. This compound also responds to lithium ion with a significant increase in fluorescence. In Phase II, we expect to synthesize more of these compounds which are likely to improve on our Phase I effort with respect to selectivity and fluorescent properties. We will also explore the use of these compounds in devising a simple spot test for determination of lithium concentration in a drop of blood. Such a test which would not require a technician to draw blood samples could easily be performed in the psychiatrist's office to determine patient compliance with his/her lithium carbonate therapy. Other applications of these compounds including ion selective electrode (ISE) preparation and synthesis of water soluble analogs for use in cell physiology studies of the role of lithium ion in inhibition of inositol phosphate metabolism and other cell mechanisms will also be explored.