MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

本提案概述了一组需要 最先进的电子顺磁共振光谱仪 研究了一些生物学问题。 的应用 所要求的文书包括：（1）结构和 黄嘌呤钼中心的机理研究 氧化酶和亚硫酸盐氧化酶的影响;（2）化学物质对 对质体蓝素的结构和功能进行修饰， 铜中心，（3）氧化还原测定 黄素氧还蛋白基因工程形式的潜力 从脱硫弧菌的，（4）底物的自旋标记研究 与黄嘌呤氧化酶结合，旨在建立 黄嘌呤与酶的氧化形式结合， 活性位点中存在的底物结合位点的数量，（5） 一氧化碳细胞色素c的激光诱导激发的应用 氧化酶和亚硝基血红蛋白检测EPR的变化 这些中心的信号，和（6）检查自旋稳定的 培养心肌细胞缺血时产生的自由基 条件，以确定在何种程度上，这种激进的发挥， 在缺血相关组织损伤中的作用。 总之，这些项目 构成电子顺磁的主要应用 共振光谱学，这将大大提高基本的 俄亥俄州一个扩大的生物化学家小组的研究能力 州立大学

The present proposal outlines a group of projects requiring a state-of-the-art electron paramagnetic resonance spectrometer in examining a number of biological problems. Applications of the instrument being requested include: (1) structural and mechanistic studies of the molybdenum centers of xanthine oxidase and sulfite oxidase, (2) studies of the effects of chemical modification of plastocyanin on the structure and function of its copper center, (3) determination of the oxidation-reduction potentials of genetically engineered forms of the flavodoxin from Desulfovibrio vulgaris, (4) a spin-label study of substrate binding to xanthine oxidase aimed at establishing the Kd for binding of xanthine to the oxidized form of the enzyme and the number of substrate binding sites present in the active site, (5) use of laser-induced excitation of carbonmonoxy cytochrome c oxidase and nitrosylhemoglobin to examine changes in the EPR signals of these centers, and (6) an examination of spin-stabilized free radicals generated by cultured heart cells under ischemic conditions to determine the extent to which such radicals play a role in ischemia-related tissue damage. Together, these projects constitute a major application of electron paramagnetic resonance spectroscopy that would greatly enhance the basic research capability of an extended group of biochemists at Ohio State University