MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

本提案概述了一组需要 最先进的电子顺磁共振波谱仪 在研究一些生物学问题时。的应用 所要求的仪器包括：(1)结构和 黄嘌呤钼中心的机理研究 氧化物酶和亚硫酸盐氧化物酶(2)化学作用的研究 藻蓝蛋白对其结构和功能的修饰 铜心，(3)氧化还原的测定 黄毒素基因工程形式的应用前景 (4)底物的自旋标记研究 与黄嘌呤氧化物酶的结合旨在建立Kd 黄嘌呤与酶的氧化形式的结合 活性部位中存在的底物结合位点数，(5) 激光诱导激发一氧化碳细胞色素c的应用 氧化物酶和亚硝酸血红蛋白检测EPR的变化 这些中心的信号，以及(6)自旋稳定化的检验 培养心肌细胞在缺血状态下产生的自由基 确定这些激进分子在多大程度上发挥作用的条件 在与缺血相关的组织损伤中的作用。这些项目加在一起， 构成电子顺磁的主要应用 共振光谱学将大大增强基本的 俄亥俄州一大批生物化学家的研究能力 州立大学

The present proposal outlines a group of projects requiring a state-of-the-art electron paramagnetic resonance spectrometer in examining a number of biological problems. Applications of the instrument being requested include: (1) structural and mechanistic studies of the molybdenum centers of xanthine oxidase and sulfite oxidase, (2) studies of the effects of chemical modification of plastocyanin on the structure and function of its copper center, (3) determination of the oxidation-reduction potentials of genetically engineered forms of the flavodoxin from Desulfovibrio vulgaris, (4) a spin-label study of substrate binding to xanthine oxidase aimed at establishing the Kd for binding of xanthine to the oxidized form of the enzyme and the number of substrate binding sites present in the active site, (5) use of laser-induced excitation of carbonmonoxy cytochrome c oxidase and nitrosylhemoglobin to examine changes in the EPR signals of these centers, and (6) an examination of spin-stabilized free radicals generated by cultured heart cells under ischemic conditions to determine the extent to which such radicals play a role in ischemia-related tissue damage. Together, these projects constitute a major application of electron paramagnetic resonance spectroscopy that would greatly enhance the basic research capability of an extended group of biochemists at Ohio State University