HEME PROTEIN MODEL STUDIES UTILIZING RUTHENIUM PORPHYRINS

该项目的长期目标是更好地了解 Ru-卟啉与血红素蛋白配基结合机理的研究 Ru取代的肌红蛋白和血红蛋白作为模型体系 天然产生的蛋白质。对氧结合的广泛理解 血红蛋白将为更好地处理遗传性疾病提供信息 涉及到血红蛋白。该项目的具体目标有两个方面。 首先，配体交换动力学、电化学和光谱 将对一系列Ru(II)卟啉进行研究，以确定 配位体与Ru的键合方式。这事很重要 为了理解这些Ru的卟啉是如何在 并入到血红蛋白中。第二，一系列的Ru取代 将制备血红素蛋白，并研究其CO和O2亲和力。 为了了解更多关于天然配基结合的机制 血红蛋白。

The long range objectives for this project are to better understand the mechanism of ligand binding in heme proteins using ruthenium porphyrins and ruthenium substituted myoglobin and hemoglobin as model systems for the naturally occurring proteins. A broad understanding of oxygen binding in hemoglobin will provide information to better deal with genetic diseases involving hemoglobin. The specific aims of the project are two fold. First, the ligand exchange kinetics, electrochemistry, and spectroscopy of a series of ruthenium(II) porphyrins will be studied in order to determine the mode of bonding of ligands to ruthenium porphyrins. This is important to an understanding of how these ruthenium porphyrins function when incorporated into hemoglobin. Second, a series of ruthenium substituted heme proteins will be prepared and their CO and O2 affinities studied in order to learn more about the mechanism of ligand binding in native hemoglobin.