INTERFACIAL DYNAMICS OF ELECTRON TRANSFER REACTIONS OF PROTEINS

蛋白质电子转移反应的界面动力学

• 批准号: 3777865
• 负责人: ANA R GUADALUPE
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3777865
• 关键词: biosensor device; chemical transfer reaction; coulometry; electrochemistry; electron transport; enzyme activity; enzyme electrodes; enzyme structure; ferrocene; ionic strengths; metal complex; oxidation reduction reaction; oxidoreductase; polymers; redoxin; surface property; thermodynamics; thin layer chromatography

The long term goal of this proposal is the development of biologically sensitive, selective and reliable electrochemical devices for the determination of metabolites of physiological relevance. At the research level, these devices can be used to study the relationship between periodic fluctuations of metabolites and the development of disorders in the human body. At the application level, the development of these devices can result in the decentralization of clinical laboratories by providing the physicians and ambulatory patients a fast, reliable, easy and low cost way of analyzing for metabolites. To achieve this goal it is necessary to understand the interfacial dynamics of electron transfer reactions of redox enzymes. What variables control the electron transfer reaction between a redox enzyme and an electrode surface? In the presence of redox mediators how fast is the electron transfer reaction between the enzyme and the mediator? What variables allow its control? We propose to continue our efforts toward the discovery of potentially useful redox mediators. We also propose to study the electron transfer reaction between these redox mediators and several redox enzymes. The enzymatic systems to be studied are oxidases such as lactate, choline and cholesterol and dehydrogenases such as malate and glycerol. Redox mediators to be tested are conductive polymers, organic metals and transition metal complexes. Electrochemical and spectroscopical techniques such as UV-Vis, Cyclic and Rotating Disk Voltammetry, Bulk Electrolysis and Spectro-electrochemistry will be used to study how variables such as pH, ionic strength, the concentration of the enzyme, cofactor, substrate and mediator and the presence of metal ions affect the kinetics and thermodynamics of these redox reactions.

该提案的长期目标是生物学的发展， 灵敏、选择性和可靠的电化学装置， 生理相关代谢物的测定。 在研究 水平，这些设备可以用来研究周期性 代谢物的波动和人类疾病的发展 身体 在应用层面，这些设备的开发可以 通过提供临床实验室， 医生和门诊病人快速，可靠，简单和低成本的方式 分析代谢物 为了实现这一目标，有必要了解界面动力学 氧化还原酶的电子转移反应。 哪些变量控制 氧化还原酶和电极之间的电子转移反应 表面？ 在氧化还原介质存在下， 酶和介体之间的转移反应？ 哪些变量 允许其控制？ 我们建议继续努力 潜在有用的氧化还原介质。 我们亦建议研究 这些氧化还原介体和几种氧化还原 内切酶 待研究的酶系统是氧化酶，如乳酸、胆碱 以及胆固醇和胆固醇酶如苹果酸和甘油。 氧化还原 待测试的介质是导电聚合物、有机金属和 过渡金属配合物 电化学和光谱技术 例如UV-Vis、循环和旋转盘伏安法、本体电解和 光谱电化学将用于研究pH值， 离子强度、酶浓度、辅因子、底物和 介体和金属离子的存在影响动力学， 这些氧化还原反应的热力学。