INTERFACIAL DYNAMICS OF ELECTRON TRANSFER REACTIONS OF PROTEINS

我们建议研究界面的电子转移。 几种蛋白质在氧化还原前后的反应 调解人。其目的是为了了解 直接电子和介电电子的能量学和动力学 蛋白质在电极表面的转移反应。要完成 为此，将进行伏安研究，以表征 正在研究的系统。光谱电化学与转盘 将使用技术来确定热力学和动力学 参数。 选择进行研究的蛋白质是因为它们的 催化生理性底物氧化的能力 和/或病理意义。这些酶反应可能 代表着伏安法未来发展的基础 可能在临床实验室中应用的传感器 诊断由异常引起的疾病和/或障碍 代谢物的浓度。

We propose to investigate the interfacial electron transfer reaction of several proteins in the absence and presence of redox mediators. The purpose is to gain an understanding of the energetics and kinetics of the direct and mediated electron transfer reaction of proteins at electrode surfaces. To accomplish this, a voltammetric study will be carried out to characterize the systems under study. Spectroelectrochemistry and rotating disk technique will be employed to determine thermodynamic and kinetic parameters, respectively. The proteins chosen for study were selected because of their ability to catalize the oxidation of substrates of physiological and/or pathological significance. These enzymatic reactions may represent the basis for the future development of voltammetric sensors that may find application in the clinical laboratory for the diagnosis of diseases and/or disorders caused by abnormal concentrations of metabolites.