INTERFACIAL DYNAMICS OF ELECTRON TRANSFER REACTIONS OF PROTEINS

蛋白质电子转移反应的界面动力学

• 批准号: 3915721
• 负责人: ANA R GUADALUPE
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3915721
• 关键词: bioenergetics; electrochemistry; electron transport; enzyme mechanism; enzyme structure; ferrocene; redoxin; thermodynamics

We propose to investigate the interfacial electron transfer reaction of several proteins in the absence and presence of redox mediators. The purpose is to gain an understanding of the energetics and kinetics of the direct and mediated electron transfer reaction of proteins at electrode surfaces. To accomplish this, a voltammetric study will be carried out to characterize the systems under study. Spectroelectrochemistry and rotating disk technique will be employed to determine thermodynamic and kinetic parameters, respectively. The proteins chosen for study were selected because of their ability to catalize the oxidation of substrates of physiological and/or pathological significance. These enzymatic reactions may represent the basis for the future development of voltammetric sensors that may find application in the clinical laboratory for the diagnosis of diseases and/or disorders caused by abnormal concentrations of metabolites.

我们建议研究界面电子转移 在存在和不存在氧化还原的情况下几种蛋白质的反应 调解员 目的是为了了解 直接电子和介导电子的能量学和动力学 蛋白质在电极表面的转移反应。 完成 为此，将进行伏安法研究以表征 研究中的系统。 光谱电化学与转盘 将采用技术来确定热力学和动力学 参数。 选择用于研究的蛋白质是因为其 催化生理活性物质底物氧化的能力 和/或病理学意义。 这些酶促反应可 代表伏安法未来发展的基础 可以在临床实验室中找到应用的传感器， 由异常引起的疾病和/或病症的诊断 代谢物的浓度。