QUADRUPOLE GAS CHROMATOGRAPH-MASS SPECTROMETER

这项提议要求支持共享四极气体 色谱质谱计仪器允许 生物材料中稳定同位素的测量。 机构资金、空间和技术支持一直是 致力于这个项目，并已经使 购买一台同位素比质谱仪。的建议。 将使用该设施的6名由PHS资助的调查人员是 已提交；4人目前正在进行由小灵通资助的稳定同位素工作。 包括：(1)格里格斯(首席调查员)，他的工作 包括全身亮氨酸动力学的测量和 ~(13)C-亮氨酸掺入骨骼肌蛋白的研究 神经肌肉疾病患者和正常人。(2)奈尔 (实验室主任)他的I型糖尿病的初步数据 患者和正常受试者提示胰岛素的主要 对蛋白质代谢的作用是减少蛋白质的降解，并且 胰高血糖素增加亮氨酸氧化。他的工作也将 检查间接计算的整体之间的关系 体内蛋白质合成与L-(1-13C)亮氨酸的掺入 转化为骨骼肌蛋白和血浆蛋白。(3)韦勒 将研究能量平衡变化对蛋白质的影响 L-(1-13C)亮氨酸作为代谢示踪剂及其对葡萄糖的影响 代谢使用6，6，2H2葡萄糖。(4)莫胥黎将研究 胰岛素对骨骼肌氨基酸代谢的影响 使用L-(1-13C)亮氨酸作为前臂研究的示踪剂 在分级的正常血糖胰岛素注射过程中。(5)阿马特鲁达和 斯帕克斯将在动物研究中使用稳定同位素直接 测量载脂蛋白的合成，并将探索其在 正常人和疾病状态下载脂蛋白的研究。 我们有在测量同位素方面有专长的调查人员 大量的血浆、呼出的空气和组织样本。同位素 我们正在获得的比率质谱仪将允许我们 测量呼出空气中13C02的同位素丰度(13C/12C) 吲哚丙三酮释放的13CO2/12C02氨基酸比值。 组织蛋白。为了继续进行血浆同位素研究 我们必须有一台气相色谱-质谱仪 具有化学电离和电子碰撞电离模式， 它可以确定负离子，并处理填充和 毛细管柱。韩礼德和马修斯的实验室 使我们目前使用稳定同位素进行研究成为可能，但 无法处理我们的表格中扩大的样本数量 调查。

This proposal requests support for a shared quadrupole gas chromatograph mass spectrometer instrument to permit the measurement of stable isotopes in biological materials. Institutional financial, space, and technical support have been committed for this project and have already made possible the purchase of an isotope ratio mass spectrometer. The proposals of 6 PHS-funded investigators who will use the facility are submitted; 4 are currently doing PHS-funded stable isotope work. Included are: (1) Griggs (Principle Investigator) whose work involves the measurement of whole body leucine kinetics and the incorporation of 13C leucine into skeletal muscle protein in patients with neuromuscular disease and in normals. (2) Nair (Laboratory Director) whose preliminary data in type I diabetic patients and normal control subjects suggest that insulin's main action on protein metabolism is to decrease proteolysis, and glucagon's to increase leucine oxidation. His work will also examine the relationship between indirectly calculated whole body protein synthesis and the incorporation of L-(1-13C) leucine into skeletal muscle protein and into plasma protein. (3) Welle will study the effect of the changes in energy balance on protein metabolism using L-(1-13C) leucine as a tracer and on glucose metabolism using 6,6 2H2 glucose. (4) Moxley will study the effect of insulin on skeletal muscle amino acid metabolism by using L-(1-13C) leucine as a tracer for forearm studies conducted during graded euglycemic insulin infusions. (5) Amatruda and Sparks will use stable isotopes in animal studies to directly measure apolipoprotein synthesis and will explore their use for studies of apolipoproteins in normal man and in disease states. We have investigators with expertise in measuring isotopic abundance in plasma, expired air, and tissue samples. The isotope ratio mass spectrometer which we are obtaining will permit us to measure the isotopic abundance (13C/12C) of 13C02 in expired air and the ninhydrin-liberated 13CO2/12C02 ratio of amino acids in tissue protein. In order to pursue studies of plasma isotopic abundance we must have a gas chromatograph mass spectrometer with chemical ionization and electron impact ionization modes, which can determine negative ions, and handle both packed and capillary columns. The laboratories of Halliday and of Matthews have made possible our current studies using stable isotopes but cannot handle the expanded number of samples form our investigations.