DIFFERENTIAL SENSITIVITY TO SEGREGATION DISTORTION IN D MELANOGASTER

这是一个常规的研究子项目，旨在确定 染色体特异性对减数分裂驱动系统分离的敏感性 黑腹果蝇的失真，并提供研究培训 旨在教授各种技术和分析的机会 技能。 A.具体目标 1.从自然界中分离出具有SD染色体特异性的染色体 敏感度。 2.通过重组来映射该属性的位置。 3.确定其分子遗传学基础。 4.分析SD可能的作用部位--Responder基因座 这些不寻常的染色体来确定它是否被改变了。 5.确定这些自然种群是否存在任何分子变异 在偏析扭曲者处，SD轨迹。 6.为2名学生提供本科生科研培训机会 学生参与研究技术，如股票构造和 维护、统计和隔离数据分析、限制 作图，Southern分析，亚克隆，可能还有DNA测序。

This is a regular research subproject intended to determine the cause of chromosome-specific sensitivity to the meiotic drive system Segregation Distortion, in Drosophila melanogaster and to provide research training opportunities designed to teach a variety of techniques and analytical skills. A. SPECIFIC AIMS 1. To isolate chromosomes from nature which exhibit SD chromosome specific sensitivity. 2. To map the location of this property by recombination. 3. To identify its molecular genetic basis. 4. To analyze the Responder locus, the putative site of action of SD, on these unusual chromosomes to determine if it has been altered. 5. To determine if these natural populations harbor any molecular variants at the Segregation distorter, Sd locus. 6. To provide undergraduate research training opportunities for 2 student participants in research techniques such as stock construction and maintenance, statistical and segregation data analysis, restriction mapping, Southern analysis, subcloning and possibly DNA sequencing.