DIFFERENTIAL SENSITIVITY TO SEGREGATION DISTORTION IN D MELANOGASTER

这是一个定期的研究子项目，旨在确定 染色体对减数分裂驱动系统的敏感性 畸变，在果蝇和提供研究培训 旨在教授各种技术和分析的机会 skills. A. 具体目标 1. 从自然界中分离具有SD染色体特异性的染色体 灵敏度 2. 通过重组来绘制这个属性的位置。 3. 以确定其分子遗传基础。 4. 为了分析反应基因座，即SD的假定作用位点， 这些不寻常的染色体来确定它是否被改变了。 5. 为了确定这些自然种群是否含有任何分子变异 在分离扭曲基因Sd位点 6. 为2名本科生提供研究培训机会 学生参与研究技术，如股票建设和 维护、统计和隔离数据分析、限制 作图、Southern分析、亚克隆和可能的DNA测序。

This is a regular research subproject intended to determine the cause of chromosome-specific sensitivity to the meiotic drive system Segregation Distortion, in Drosophila melanogaster and to provide research training opportunities designed to teach a variety of techniques and analytical skills. A. SPECIFIC AIMS 1. To isolate chromosomes from nature which exhibit SD chromosome specific sensitivity. 2. To map the location of this property by recombination. 3. To identify its molecular genetic basis. 4. To analyze the Responder locus, the putative site of action of SD, on these unusual chromosomes to determine if it has been altered. 5. To determine if these natural populations harbor any molecular variants at the Segregation distorter, Sd locus. 6. To provide undergraduate research training opportunities for 2 student participants in research techniques such as stock construction and maintenance, statistical and segregation data analysis, restriction mapping, Southern analysis, subcloning and possibly DNA sequencing.