CIRCULAR DICHROISM INSTRUMENTATION

请拨经费购买圆二向色性(CD)光谱偏振仪。 北卡罗来纳大学没有CD乐器。大多数实验都提出了 包括通过观察蛋白质的二级结构来检查蛋白质的二级结构 棉花在紫外线中的作用。即将出现的蛋白质和多肽 研究内容包括：(1)酵母菌的天然和变性变种 酿酒酵母等-1-细胞色素c，(2)含有γ-的凝血蛋白- 二价金属离子存在和不存在时的羧基谷氨酸 和/或带负电荷的磷脂，(3)核糖体及其 辅助因子，(4)凝血调节蛋白和多肽 系统中存在和不存在糖胺聚糖，(5)非遗传性 合成具有特殊结构和化学性质的蛋白质 (6)超薄多肽-硅胶结构。线性二色性将 也要检查超薄的多肽-二氧化硅结构。 紫外圆二色谱将用于指认绝对构型 过渡金属络合物在高纯合成中的重要作用 用于生物技术的分子。将检查可见区域中的CD 确定手性金属有机化合物的绝对构型 用于对映选择性有机反应的络合物。可见的CD 近红外区以及磁性CD将被检查以 确定细胞色素c变异体的血红素环境 Co(II)取代胞苷活性中心几何构型的研究 脱氨酶。

Funds are requested for a circular dichroism (CD) spectropolarimeter. There is no CD instrument at UNC-CH. Most of the experiments proposed involve examination of protein secondary structure by observation of Cotton effects in the ultraviolet. The proteins and peptides to be studied include: (1) native and denatured variants of Saccharomyces cerevisiae iso-1-cytochrome c, (2) coagulation proteins containing gamma- carboxy glutamic acid in the presence and absence of divalent metal ions and/or negatively-charged phospolipids, (3) ribosomes and their co-factors, (4) regulatory proteins and peptides of the coagulation system in the presence and absence glycosaminglycans, (5) non-genetic proteins synthesized to have special structural and chemical properties and (6) ultrathin polypeptide-silica structures. Linear dichroism will also be examined for the ultrathin polypeptide-silica structures. Ultraviolet CD spectra will be used to assign absolute configurations of transition metal complexes of importance for synthesis of highly-pure molecules for biotechnology. CD in the visible region will be examined to determine the absolute configuration of chiral organometallic complexes used for enantioselective organic reactions. CD in the visible and near infrared regions as well as magnetic CD will be examined to determine the heme environment of cytochrome c variants and for investigation of the active site geometry of Co(II)-substituted cytidine deaminase.