OPTIMA XL-A ANALYTICAL ULTRACENTRIFUGE SYSTEM

OPTIMA XL-A 分析超速离心系统

• 批准号: 3521645
• 负责人: MARC Wallace KIRSCHNER
• 金额: $ 12.4万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-24 至 1993-06-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521645
• 关键词: analytical ultracentrifugation; biomedical equipment purchase; molecular weight; protein structure function

We propose to purchase a Beckman Optima XL-A Analytical Ultracentrifuge to serve as a Departmental facility in the UCSF Department of Biochemistry and Biophysics. The Department contains more than 40 active research groups, and we anticipate that this ultracentrifuge will find wide use throughout the Department. It will be placed in a shared centrifuge facility that presently houses preparative ultracentrifuges. The cost of maintaining and servicing the instrument will be recharged by the Department to the individual laboratories according to use. The instrument will be overseen by Chris Field, who has been a Staff Research Associate in the Alberts and Kirschner laboratories for more than ten years. The major users listed on the grant are Marc Kirschner, whose project is entitled "Oligomerization of Tubulins and Cell Cycle Proteins;" Bruce Alberts ("Isolation and Characterization of Multiprotein Complexes"); Tim Mitchison ("Microtubule Dynamics and Mitotic Mechanism"); and Alexander (Sandy) Johnson ("Combinatorial Control of Gene Expression in Yeast"). Both Alberts and Kirschner have made extensive use of the old Beck" Model E Analytical Ultracentrifuge in their past scientific work, and both have taught the theory and practice of hydrodynamic measurements on macromolecules in graduate school courses. The role that this type of instrument plays in biology has decreased considerably in the past dime decades. But the central issues in biology have changed in recent years. After a very productive phase in which powerful new methods have been used to identify and isolate many new proteins, we now need to focus on figuring out how these proteins work in the cell. Since most proteins function in protein complexes, we need powerful ways of deciphering macromolecular interactions. Analytical ultracentrifugation is an invaluable technique for determining native molecular weights, and it is also a powerful way of measuring conformational changes, densities, macromolecule heterogeneity, and ligand binding to proteins. At UCSF, the environment in which the new ultracentrifuge will be kept will make it available to a large community. Moreover, to make students, postdoctoral fellows, and faculty aware of the potential of this instrument, hydrodynamic theory will be taught in our required Structural Biology course for first year graduate students. In addition, we will include descriptions of advanced techniques using analytical ultracentrifugation as part of our on-going Special Topics Course taught on Saturdays.

我们建议购买Beckman Optima XL-A分析型超声心动图， 作为UCSF生物化学系的部门设施， 生物物理学 该部有40多个活跃的研究小组， 我们预计，这种超声波将广泛用于 本部门 它将被放置在一个共享的离心机设施中， 目前存放着制备型超浓缩细菌 维护成本和 维修仪器的费用将由本署向 实验室根据使用情况。 该仪器将由 克里斯·菲尔德（Chris Field），他曾是阿尔伯特大学的研究助理， Kirschner实验室十多年。 列出的主要用户 获得资助人是马克·基尔施纳，他的项目名为“低聚化 微管蛋白和细胞周期蛋白;”布鲁斯艾伯茨（“分离和 多蛋白复合物的表征”）;蒂姆·米奇森（“微管 动力学和有丝分裂机制”）;和亚历山大（桑迪）约翰逊 （“酵母中基因表达的组合控制”）。 艾伯茨和基什内尔都广泛地使用了旧的贝克模型 在他们过去的科学工作中， 教授水动力测量的理论和实践， 研究生院课程中的大分子。 这种类型的角色 在过去的十年里，生物学中的乐器演奏已经大大减少了。 几十年 但近年来，生物学的核心问题发生了变化。 经过一个非常富有成效的阶段，其中强大的新方法已被使用 为了识别和分离许多新的蛋白质，我们现在需要专注于计算 这些蛋白质在细胞中是如何工作的。 由于大多数蛋白质的功能， 蛋白质复合物，我们需要强大的方法来破译大分子 交互. 分析超浓缩是一种非常宝贵的技术 用于确定天然分子量，也是一种有效的方法 测量构象变化、密度、大分子异质性， 和与蛋白质结合的配体。 在加州大学旧金山分校，新的超级计算机将被保存的环境将 让它可以被一个大的社区使用。 此外，为了使学生， 博士后研究员和教师意识到这一潜力 仪器，流体动力学理论将在我们所需的结构 研究生一年级生物学课程。 此外，我们将 包括使用分析的高级技术的描述 作为我们正在进行的专题课程的一部分， 星期六则