OPTIMA XL-A ANALYTICAL ULTRACENTRIFUGE SYSTEM

我们建议购买Beckman Optima XL-A分析型超速离心机 作为加州大学旧金山分校生物化学系的部门设施 生物物理学。该部门有40多个活跃的研究小组， 我们预计这种超离心机将在全球范围内得到广泛应用 警局。它将被放置在一个共享的离心机设施中， 目前有备用型超速离心机。维护和维护的成本 维修该仪器的费用将由本署向 个别实验室根据用途而定。该仪器将受到监督 克里斯·菲尔德，他一直是艾伯特省和 克尔施纳实验室工作了十多年。上列出的主要用户 该资助者是Marc Kirschner，他的项目名为《寡聚化 微管蛋白和细胞周期蛋白；《Bruce Alberts(分离和 多蛋白复合体的特征“)；Tim Mitchison(”微管 《动力学和有丝分裂机制》)；亚历山大(桑迪)约翰逊 (“酵母基因表达的组合控制”)。 艾伯茨和基什内尔都广泛使用了旧的贝克模式 E分析超速离心机在他们过去的科学工作中，而且两人都 讲授水动力测量的理论和实践 研究生院课程中的大分子。这种类型的角色 在过去的十年里，生物学中的乐器演奏大大减少了 几十年。但近年来，生物学的核心问题已经发生了变化。 在使用了强大的新方法的非常富有成效的阶段之后 为了鉴定和分离许多新的蛋白质，我们现在需要专注于计算 了解这些蛋白质在细胞中是如何工作的。因为大多数蛋白质在 蛋白质复合体，我们需要强大的方法来破译大分子 互动。分析超速离心法是一项非常有价值的技术。 用于确定天然分子量，这也是一种强大的方法 测量构象变化，密度，大分子异质性， 以及与蛋白质结合的配体。 在加州大学旧金山分校，保存新的超速离心机的环境将 让它在一个大社区中可用。此外，为了让学生， 博士后研究员和教职员工意识到了这一潜力 仪器、流体力学理论将在我们要求的结构中讲授 为研究生一年级开设的生物学课程。此外，我们还将 包括使用分析工具描述高级技术 超速离心法作为我们正在进行的专题课程的一部分 在周六。

We propose to purchase a Beckman Optima XL-A Analytical Ultracentrifuge to serve as a Departmental facility in the UCSF Department of Biochemistry and Biophysics. The Department contains more than 40 active research groups, and we anticipate that this ultracentrifuge will find wide use throughout the Department. It will be placed in a shared centrifuge facility that presently houses preparative ultracentrifuges. The cost of maintaining and servicing the instrument will be recharged by the Department to the individual laboratories according to use. The instrument will be overseen by Chris Field, who has been a Staff Research Associate in the Alberts and Kirschner laboratories for more than ten years. The major users listed on the grant are Marc Kirschner, whose project is entitled "Oligomerization of Tubulins and Cell Cycle Proteins;" Bruce Alberts ("Isolation and Characterization of Multiprotein Complexes"); Tim Mitchison ("Microtubule Dynamics and Mitotic Mechanism"); and Alexander (Sandy) Johnson ("Combinatorial Control of Gene Expression in Yeast"). Both Alberts and Kirschner have made extensive use of the old Beck" Model E Analytical Ultracentrifuge in their past scientific work, and both have taught the theory and practice of hydrodynamic measurements on macromolecules in graduate school courses. The role that this type of instrument plays in biology has decreased considerably in the past dime decades. But the central issues in biology have changed in recent years. After a very productive phase in which powerful new methods have been used to identify and isolate many new proteins, we now need to focus on figuring out how these proteins work in the cell. Since most proteins function in protein complexes, we need powerful ways of deciphering macromolecular interactions. Analytical ultracentrifugation is an invaluable technique for determining native molecular weights, and it is also a powerful way of measuring conformational changes, densities, macromolecule heterogeneity, and ligand binding to proteins. At UCSF, the environment in which the new ultracentrifuge will be kept will make it available to a large community. Moreover, to make students, postdoctoral fellows, and faculty aware of the potential of this instrument, hydrodynamic theory will be taught in our required Structural Biology course for first year graduate students. In addition, we will include descriptions of advanced techniques using analytical ultracentrifugation as part of our on-going Special Topics Course taught on Saturdays.