STUDIES ON DNA CONDENSATION AND PROTEIN CONFORMATION

DNA 缩合和蛋白质构象的研究

• 批准号: 3519507
• 负责人: CARLOS Jose BUSTAMANTE
• 金额: $ 6.9万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-05-15 至 1987-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519507
• 关键词: biomedical facility; circular dichroism; fluorescence; optical rotation; spectrometry

This is a proposal to obtain a modern multi-optional spectro polarimeter for the Chemisty Department of the University of New Mexico. This instrument will have the capabilities to perform 1) magnetic circular dichroism, 2) fluorescence-detected circular dichroism (FDCD), 3) fast scanning and averaging of large number of spectra, 4) large data storage accessories and specialized software for data processing, 5) low-temperature capabilities, and 6) stopped flow accessory for kinetic studies. The three main users of this instrument will be Dr. Carlos Bustamante (Chemistry Department), Dr. Mark R. Ondrias (Chemistry Department), and Dr. David G. Bear (Cell Biology Department). Dr. Bustamante proposes to study and characterize the condensation of DNA in vitro by following the appearance of the so-called Psi-type CD anomalies, observed in compacted forms of DNA. Recently, a theory of these effects has been developed in Dr. Bustamante's laboratory. Dr. Mark R. Ondrias is currently studying the transient and steady state Raman spectra of multi-heme proteins. Dr. Ondrias proposes to characterize the state of oxidation of trapped photolytic transients via magnetic circular dichroism spectroscopy. Dr. David G. Bear proposes to study the binding of the transcription termination factor rho to specific messenger RNA by following the changes induced in the circular dichroism upon binding. Each co-P.I. will have access to 25% of the total available user time of the instrument. In addition, this proposal contains the contribution of Professor Fritz S. Allen as a user outside of the main user group. Dr. Allen plans to study the molecular mechanisms of allosteric regulation of liver phosphofructo-kinase, by conducting stopped-flow studies and following the CD changes that accompany NAD/NADH conversion.

这是一个获得现代多选择光谱偏振计的建议 在新墨西哥州大学化学系工作。 这 仪器将有能力执行1）磁性循环 二色性，2）荧光检测圆二色性（FDCD），3）快速 大量光谱的扫描和平均，4）大数据存储 附件和数据处理专用软件，5） 低温能力，以及6）用于动力学的止流附件 问题研究 这台仪器的三个主要用户将是卡洛斯博士 Bustamante（化学系），Dr. Ondrias（化学 部门）和大卫·G博士。熊（细胞生物学系）。 博士 布斯塔曼特建议研究和表征DNA在 通过观察所谓的Psi型CD异常的出现， 在DNA的压缩形式中观察到。 最近，一个关于这些效应的理论 是在布斯塔曼特博士的实验室里研发出来的 马克·R博士翁德里亚斯群岛 目前正在研究的瞬态和稳态拉曼光谱的 多血红素蛋白 Ondrias博士建议将 通过磁性圆二色性捕获光解瞬变的氧化 谱 大卫博士G.贝尔建议研究 转录终止因子rho与特异性信使RNA结合， 结合时引起的圆二色性的变化。 每一个合作PI 将可以访问25%的总可用用户时间， 仪器 此外，本提案还载有下列方面的贡献： Fritz S.艾伦作为主用户组之外的用户。 博士 艾伦计划研究变构调节的分子机制， 肝磷酸果糖激酶，通过进行停流研究， CD的变化伴随着NAD/NADH的转换。