LASER FACILITY FOR BIOPHYSICAL STUDIES

用于生物物理研究的激光设备

• 批准号: 3519447
• 负责人: JAMES C MARTIN
• 金额: $ 29.8万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-02-01 至 1987-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519447
• 关键词: Raman spectrometry; argon; biophysics; fluorescence; fluorescence spectrometry; lasers; light scattering

Support is requested for a laser based facility for the joint use of a group of investigators in the biophysical and biomedical sciences. Two argon ion lasers systems capable of providing combinations of continuous wave and picosecond pulsed output in the visible and ultraviolet will provide the core of this instrumentation. Requested equipment will allow access to four important experimental techniques which are currently required by local NIH funded investigators, but not available within the state. These techniques are fluorescence recovery after photobleaching (with imaging), picosecond time resolved fluorescence spectroscopy, dynamic light scattering, and resonance Raman spectroscopy. Specific plans are given to use these techniques to study ion channels in membranes, drug interactions with cells, cell-cell interactions, mechanisms of calcium regulation in muscle contraction, protein-calcium interactions, structures of lipid/protein micellar complexes, and conformational microheterogeneity of DNA. Significant saving in acquisition costs, opportunities for enhanced scientific collaboration and the availability of personnel with significant experience with lasers will result from the shared nature of this proposed facility.

请求支持基于激光的设施，以便联合使用 生物物理和生物医学领域的研究人员小组。 二 氩离子激光器系统能够提供连续的组合 可见光和紫外光波和皮秒脉冲输出 提供该仪器的核心。 请求的设备将允许 获得目前正在使用的四种重要的实验技术 当地 NIH 资助的研究人员要求，但在 状态。 这些技术是光漂白后的荧光恢复 （带成像），皮秒时间分辨荧光光谱，动态 光散射和共振拉曼光谱。 具体计划是 给予使用这些技术来研究膜中的离子通道，药物 与细胞的相互作用、细胞与细胞的相互作用、钙的机制 肌肉收缩、蛋白质-钙相互作用、结构的调节 脂质/蛋白质胶束复合物和构象微观异质性 DNA。 显着节省购置成本、机会 加强科学合作和人员配备 激光方面的重要经验将源于以下方面的共同性质： 这个拟议的设施。