KRATOS MS-80 MASS SPECTROMETER

一种中等质量范围的扇形磁质谱计， 分辨率要求满足霍姆伍德用户组的需求 约翰霍普金斯大学的化学系， 生物学和环境健康科学。 一系列化工项目 生物学意义：（1）对该菌的生物合成研究 黄曲霉毒素，环境真菌毒素，以及经典和 非经典β-内酰胺类抗生素 (2)的不对称合成 对映体纯的手性化合物和天然产物，新的合成 碳环的构建和多环的修饰方法 芳烃转化为亲电烷基化剂。 （三） 表征合成糖缀合物，包括中间体和 降解产物，这将广泛用于研究 受体介导的碳水化合物内吞作用。 (4)为了鉴别烷基化的 来自酶激活底物抑制的酶的氨基酸 脱氨反应机理的方法和示踪研究。 （五） 日光刺激黑素生成的分子基础研究 以及这种刺激和病理状态之间的相互作用 恶性黑色素瘤

A magnetic sector mass spectrometer of intermediate mass range and resolution is requested to meet the needs of a user group on the Homewood campus of the Johns Hopkins University from the Departments of Chemistry, Biology and Environmental Health Sciences. A range of projects of chemical and biological significance is described: (1) Biosynthetic studies of the aflatoxins, environmental mycotoxins, and of the classical and non-classical Beta-lactam antibiotics. (2) Asymmetric syntheses of enantiomerically pure synthons and natural products, new synthetic methdology for construction of carbocycles and modification of polycyclic aromatic hydrocarbons into electrophilic alkylating agents. (3) Characterizing synthetic glycoconjugates, including intermediates and degradation products, which will be used extensively in studies of receptor-mediated endocytosis of carbohydrates. (4) To identify alkylated amino acids from enzymes inhibited by the enzyme-activated-substrate approach and tracer studies of the mechanism of deamination reactions. (5) Investigations on the molecular basis of sunlight-stimulated melanogenesis and the interplay between this stimulation and the pathological condition of malignant melanoma.