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500 MHZ NMR INSTRUMENTATION FOR SHARED USE

供共享使用的 500 MHz NMR 仪器

基本信息

批准号：
3521167
负责人：
CRAIG ARTHUR TOWNSEND
金额：
$ 40万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-04-15 至 1992-10-14
项目状态：
已结题

项目摘要

A high field (500 MHz), multinuclear spectrometer is requested to meet the needs of a user group at the Homewood campus of The Johns Hopkins University from the Departments of Chemistry and Biology. A range of projects of chemical and biological significance is described: (1) Biosynthetic studies of aflatoxins and of Beta-lactam antibiotics, interactions of calicheamicin with oligonucleotides. (2) Synthetic and structural investigations of oligosaccarides, glycopeptides and glycolipids for research in carbohydrate recognition and hepatic receptor binding studies. (3) Structural studies of metal-binding peptides of the "zinc finger" type and related retroviral proteins. (4) Investigation of RNA folding and RNA-protein interactions. (5) Development of new synthetic methods and their application to total asymmetric natural product syntheses. (6) Chemical modeling of oxygen- binding and oxygen-activating copper protein active sites and investigations of the metal-binding properties of glutamyl-cysteinyl . peptides. (7) Structural studies of DNA molecules and oligonucleotides with adenine tracts to understand the origins of DNA bending. (8) Identification by NMR techniques of the labeled sites of alpha- chymotrypsin inhibited with 13C-enriched enzyme-activated inhibitors. Examination of chemi- and bioluminescent reaction mechanisms. Study by low-temperature NMR of deaminatively formed carbonium ions. (9) Oxygenation of hindered and deformed olefins and the reactions and rearrangements of carbenes will be investigated. (10) Synthesis of prostaglandins and their aza-analogues. (11) Studies of the mechanism of action and design of inhibitors for human placental aromatase and lanosterol 14alpha-demethylase.

要求一台高场(500 MHz)多核谱仪满足约翰·霍普金斯大学霍姆伍德校区用户群体的需求从化学系和生物系毕业。一系列描述了具有化学和生物意义的项目：(1) 黄曲霉毒素和β-内酰胺类抗生素的生物合成研究，金雀花素与寡核苷酸的相互作用。(2)合成的和低聚糖、糖肽和低聚糖的结构研究糖脂在碳水化合物识别和肝脏研究中的应用受体结合研究。(3)金属结合的结构研究 “锌指”型多肽和相关的逆转录病毒蛋白。(4) RNA折叠和RNA-蛋白质相互作用的研究。(5) 新合成方法的发展及其在TOTAL中的应用不对称天然产物合成。(6)氧的化学模拟- 结合和氧活化铜蛋白活性部位和谷氨酰半胱氨基与金属结合性能的研究。多肽。(7)DNA分子和寡核苷酸的结构研究用腺嘌呤肌束来了解DNA弯曲的起源。(8) 用核磁共振技术鉴定α-核糖核酸的标记位点用富含13C的酶激活抑制剂抑制胰凝乳酶。化学发光和生物发光反应机理的研究。学习方式：脱氨基生成的碳正离子的低温核磁共振。(9) 受阻和变形的烯烃的氧合作用及其反应和卡宾的重排将被调查。(10)合成前列腺素及其氮杂类似物。(十一)心力衰竭发病机制研究人胎盘芳香酶和人胎盘芳香酶抑制剂的作用和设计羊毛甾醇14α-脱甲基酶。