REQUEST FOR A 500 MHZ MULTI-NUCLEAR NMR SPECTROMETER

一种最先进的多核核磁共振仪器，工作在磁场中 一个由8个主要用户和19个次要用户组成的组需要11.8特斯拉。 在主要使用者中，艾森博士建议对普鲁卡因进行质子核磁共振研究 子宫铁蛋白，希望将金属-配体之间的相互作用 活性部位对蛋白质的生化活性有影响。佩萨奇博士会 在他对血红素蛋白的研究中使用核磁共振来确定 硫化血红蛋白和硫化肌红蛋白中的修复基。阿特金森博士用 核磁共振用于N-连接复合碳水化合物的比较结构分析 动物细胞表面膜和胞内膜中的糖蛋白 和病毒。斯坦利博士从事糖基化研究。 中国仓鼠卵巢细胞突变体表现出的缺陷 细胞表面碳水化合物的结构变化。质子核磁共振是 用于获得完整的糖组成、连锁特性 以及从25-50毫摩尔的纯化的糖的部分或全部序列 糖肽。结构特征的碳水化合物被用来搜索 对于可能为每个突变体提供基础的特定酶损伤 基因分型。布鲁尔博士将继续他对 简单和复杂的碳水化合物与刀豆蛋白A，并将延长它们 小扁豆和豌豆凝集素，以深入了解糖的结合 凝集素的活性及其与细胞膜的相互作用。Dr。 考本的研究旨在表征构象特征 生物活性所需的胰岛素和松弛素，使用详细的 天然激素的质子核磁共振构象分析及其 活跃和不活跃的类似物。法布里博士正在研究 用异常红检测细胞成分中的异常血红蛋白 异质性已通过等密度离心法解决的细胞。 她还用核磁共振研究了反应的产物和机理。 葡萄糖-6P和葡萄糖(糖尿病患者红细胞中发现升高)和 乙醛(酒精中毒时红细胞中含量升高)与血红蛋白。 古普塔博士建议对各种细胞和组织进行核磁共振研究。他在寻找 了解金属离子在细胞中可能的调节作用 细胞的增殖、分化、体积调节和激素调控 不同的细胞过程，以了解浓度是如何 细胞内离子是通过膜运输过程来管理的，以及如何 这样的规定在癌症、高血压、 糖尿病和镰状细胞疾病。

A state-of-the-art multi-nuclear NMR instrument operating at a magnetic field of 11.8 Tesla is needed for a group of 8 major and 19 minor users. Of the major users, Dr. Aisen proposes proton NMR studies of procine uteroferrin, with the hope of relating metal-ligand interactions at the active site to the biochemical activity of the protein. Dr. Peisach will use NMR in his studies of hemoproteins to determine the structure of the prosthetic group in sulfhemoglobin and sulfmyoglobin. Dr. Atkinson used NMR for comparative structural analyses of N-linked complex carbohydrates in glycoproteins from surface and intracellular membranes of animal cells and viruses. Dr. Stanley is engaged in investigations of glycosylation defects exhibited by Chinese hamster ovary cell mutants shown to possess structural alterations in carbohydrates at the cell surface. Proton NMR is used to obtain the complete sugar composition, the linkage specificities and partial or complete sequence of sugars from 25-50 nmole of a purified glycopeptide. Structurally characterized carbohydrates are used to search for specific enzyme lesions which might provide the basis of each mutant genotype. Dr. Brewer will continue his studies of the interactions of simple and complex carbohydrates with Concanavalin A and will extend them to lentil and pea lectins to obtain insights into the saccharide binding activities of the lectins and their interactions with cell membranes. Dr. Cowburn's research is aimed at characterizing the conformational features of insulin and relaxin required for bioactivity, using detailed conformational analyses, by proton NMR, of native hormones, and their active and inactive analogs. Dr. Fabry is studying interactions of abnormal hemoglobins with the cellular constituents using abnormal red cells whose heterogeneity has been resolved by isopycnic centrifugation. She also uses NMR to study the products and mechanism of reaction of glucose-6P and glucose (found elevated in red cells in diabetes) and acetaldehyde (found elevated in red cells in alcoholism) with hemoglobin. Dr. Gupta proposes NMR studies of a variety of cells and tissues. He seeks to understand possible regulatory roles of metal ions in cellular proliferation, differentiation, volume regulation, and hormonal control of various cellular processes, to understand how the concentrations of intracellular ions are managed by membrane transport processes, and how such regulation goes astray in disorders such as cancer, hypertension, diabetes, and sickle cell disease.