REQUEST FOR A 500 MHZ MULTI-NUCLEAR NMR SPECTROMETER

请求 500 MHZ 多核核磁共振波谱仪

• 批准号: 3519102
• 负责人: Raj K Gupta
• 金额: $ 30万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-02-01 至 1987-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519102
• 关键词: carbohydrate structure; chemical structure function; conformation; hemoglobin; hemoprotein structure; ions; iron metabolism; magnesium; nuclear magnetic resonance spectroscopy; sodium; transferrin

A state-of-the-art multi-nuclear NMR instrument operating at a magnetic field of 11.8 Tesla is needed for a group of 8 major and 19 minor users. Of the major users, Dr. Aisen proposes proton NMR studies of procine uteroferrin, with the hope of relating metal-ligand interactions at the active site to the biochemical activity of the protein. Dr. Peisach will use NMR in his studies of hemoproteins to determine the structure of the prosthetic group in sulfhemoglobin and sulfmyoglobin. Dr. Atkinson used NMR for comparative structural analyses of N-linked complex carbohydrates in glycoproteins from surface and intracellular membranes of animal cells and viruses. Dr. Stanley is engaged in investigations of glycosylation defects exhibited by Chinese hamster ovary cell mutants shown to possess structural alterations in carbohydrates at the cell surface. Proton NMR is used to obtain the complete sugar composition, the linkage specificities and partial or complete sequence of sugars from 25-50 nmole of a purified glycopeptide. Structurally characterized carbohydrates are used to search for specific enzyme lesions which might provide the basis of each mutant genotype. Dr. Brewer will continue his studies of the interactions of simple and complex carbohydrates with Concanavalin A and will extend them to lentil and pea lectins to obtain insights into the saccharide binding activities of the lectins and their interactions with cell membranes. Dr. Cowburn's research is aimed at characterizing the conformational features of insulin and relaxin required for bioactivity, using detailed conformational analyses, by proton NMR, of native hormones, and their active and inactive analogs. Dr. Fabry is studying interactions of abnormal hemoglobins with the cellular constituents using abnormal red cells whose heterogeneity has been resolved by isopycnic centrifugation. She also uses NMR to study the products and mechanism of reaction of glucose-6P and glucose (found elevated in red cells in diabetes) and acetaldehyde (found elevated in red cells in alcoholism) with hemoglobin. Dr. Gupta proposes NMR studies of a variety of cells and tissues. He seeks to understand possible regulatory roles of metal ions in cellular proliferation, differentiation, volume regulation, and hormonal control of various cellular processes, to understand how the concentrations of intracellular ions are managed by membrane transport processes, and how such regulation goes astray in disorders such as cancer, hypertension, diabetes, and sickle cell disease.

一个国家的最先进的多核核磁共振仪器运行在一个磁 一组8个主要用户和19个次要用户需要11.8特斯拉的磁场。 在主要用户中，Aisen博士建议对猪进行质子核磁共振研究 子宫铁蛋白，与有关的金属配体相互作用的希望，在 活性位点对蛋白质的生物化学活性的影响。 Peisach博士将 在血红素蛋白的研究中使用核磁共振来确定 硫血红蛋白和硫肌红蛋白中的辅基。 阿特金森医生用 NMR用于N-连接的复杂碳水化合物的比较结构分析 动物细胞表面和细胞内膜糖蛋白中 和病毒。 斯坦利博士从事糖基化研究 中国仓鼠卵巢细胞突变体显示的缺陷， 细胞表面碳水化合物的结构改变。 质子nmr 用于获得完整的糖组成，连接特异性 和来自25-50纳摩尔的纯化的 糖肽 结构特征的碳水化合物用于搜索 可能提供每个突变体的基础的特定酶损伤 基因型 布鲁尔博士将继续研究 简单和复杂的碳水化合物与伴刀豆球蛋白A，并将延长他们 小扁豆和豌豆凝集素，以了解糖结合 凝集素的活性及其与细胞膜的相互作用。 博士 Cowburn的研究旨在表征构象特征 胰岛素和松弛素所需的生物活性，使用详细的 构象分析，通过质子核磁共振，天然激素，和他们的 活性和非活性类似物。 法布里博士正在研究 异常血红蛋白与细胞成分使用异常红 通过等密度离心分离其异质性已被解决的细胞。 她还利用核磁共振研究了反应的产物和反应机理。 葡萄糖-6 β和葡萄糖（在糖尿病红细胞中发现升高）， 乙醛（酒精中毒时红细胞中乙醛含量升高）与血红蛋白有关。 博士古普塔提出了各种细胞和组织的核磁共振研究。 他寻求 为了了解金属离子在细胞内可能的调节作用， 增殖、分化、体积调节和激素控制 各种细胞过程，以了解如何浓度 细胞内离子是由膜转运过程管理的，以及如何 这种调节在诸如癌症，高血压， 糖尿病和镰状细胞病。

项目成果

Concanavalin A interactions with asparagine-linked glycopeptides. Bivalency of bisected complex type oligosaccharides.

刀豆球蛋白 A 与天冬酰胺连接的糖肽相互作用。

• 发表时间: 1987
• 期刊: The Journal of biological chemistry
• 作者: Bhattacharyya,L;Haraldsson,M;Brewer,CF
• 通讯作者: Brewer,CF

Concanavalin A interactions with asparagine-linked glycopeptides. Bivalency of high mannose and bisected hybrid type glycopeptides.

刀豆球蛋白 A 与天冬酰胺连接的糖肽相互作用。

• 发表时间: 1987
• 期刊: The Journal of biological chemistry
• 作者: Bhattacharyya,L;Ceccarini,C;Lorenzoni,P;Brewer,CF
• 通讯作者: Brewer,CF

Interactions of asparagine-linked carbohydrates with concanavalin A. Nuclear magnetic relaxation dispersion and circular dichroism studies.

天冬酰胺连接的碳水化合物与刀豆球蛋白 A 的相互作用。核磁弛豫色散和圆二色性研究。

• 发表时间: 1991
• 期刊: The Journal of biological chemistry
• 作者: Bhattacharyya,L;Koenig,SH;Brown3rd,RD;Brewer,CF
• 通讯作者: Brewer,CF

Interactions of concanavalin A with glycoproteins. A quantitative precipitation study of concanavalin A with the soybean agglutinin.

• DOI: 10.1016/s0008-6215(00)90599-8
• 发表时间: 1991-06
• 期刊: Carbohydrate research
• 影响因子: 3.1
• 作者: M. I. Khan;D. Mandal;C. Brewer

Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glycoproteins using the phenol-sulfuric acid method.

• DOI: 10.1016/0008-6215(94)84249-3
• 发表时间: 1994-02
• 期刊: Carbohydrate research
• 影响因子: 3.1
• 作者: Anat K. Saha;Curtis F. Brewer