NMR STUDIES OF METAL IONS IN INTACT CELLS AND TISSUES

完整细胞和组织中金属离子的核磁共振研究

• 批准号: 3152403
• 负责人: Raj K Gupta
• 金额: $ 18.72万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3152403
• 关键词: bleomycin; carbonate dehydratase; chemical structure function; conformation; cytochrome c; diphosphoglycerate; enzyme substrate complex; erythrocytes; heme; hemoglobin; hormone regulation /control mechanism; human tissue; hypertension; insulin; ion transport; lymphocyte; metalloproteins; nuclear magnetic resonance spectroscopy; oxyhemoglobin; phosphotransferases; sickle cell anemia

Intracellular metal ions modulate a variety of cellular and tissue functions. A long range goal of our research is to understand possible regulatory roles of these ions in cellular proliferation, differentiation, volume regulation, and hormonal control of various cellular processes. We seek also to understand how the concentrations of intracellular ions are managed by various membrane transport processes, and how such regulation goes astray in disorders such as cancer, hypertension, diabetes, and sickle cell disease. Our primary research tool is NMR spectroscopy. We have recently developed new NMR approaches for measuring free Mg++ (by analyzing 31P NMR of intracellular ATP) and Na+ ions (using an anionic paramagnetic reagent Dy(PPPi)2 introduced by us to distinguish between intra- and extracellular Na+ ions). The choice of cells and tissues for our research includes human normal and sickle erythrocytes, peripheral blood lymphocytes, isolated rat cardiac myocytes, amphibian eggs, and rat tail artery. Specific aims to be pursued are: (1) To determine if bound Na+ exists, and is functionally significant, by quantitating NMR-visible and total intracellular Na+ ion concentrations in various types of cells and physiological states; (2) If indeed a sizable part of intracellular Na+ in human erythrocytes is NMR-invisible as indicated by our preliminary experiments, to determine if it is sequestered in the cell membrane and cytoskeleton, and whether it can be released by intracellular Ca++ ions; (3) To study localization of Na+ ions in various intracellular compartments in amphibian oocytes; (5) To find out if partially relaxed 23Na FT NMR spectra can be used to detect the existence of compartments with differing relaxation behaviour and magnetic environments within an intact cell; (5) To follow changes in [Nai], pHi, [Mgi2+] during mitotic cell division cycles in synchronously dividing amphibian oocytes; (6) To ascertain if an abnormality in the regulation of intracellular Na+ ions is associated with the disordered mitotic rate and maturation sequence of leukemic lymphocytes; (7) To characterize the state of Na+ and K+ ions in normal and sickle red blood cells in oxygenated and deoxygenated states; (8) To investigate the effects of insulin upon membrane systems involved in the transport of Na+, K+ and H+ ions in amphibian oocytes and mammalian cardiac myocytes and to determine whether such effects are mediated by proteolytically generated peptides; and (9) To test the hypothesis that an increased NMR-visible intracellular Na+ is associated with essential hypertension.

细胞内金属离子调节多种细胞和组织

项目成果

23Na NMR studies of rat outer medullary kidney tubules.

大鼠外髓肾小管的 23Na NMR 研究。

• 发表时间: 1985
• 期刊: The Journal of biological chemistry
• 作者: Rayson,BM;Gupta,RK
• 通讯作者: Gupta,RK

Steroids, intracellular sodium levels, and Na+/K+-ATPase regulation.

类固醇、细胞内钠水平和 Na /K -ATP 酶调节。

• 发表时间: 1985
• 期刊: The Journal of biological chemistry
• 作者: Rayson,BM;Gupta,RK
• 通讯作者: Gupta,RK

Studies on the mechanism of decreased NMR-measured free magnesium in stored erythrocytes.

研究核磁共振测量的储存红细胞中游离镁减少的机制。

• DOI: 10.1016/0167-4889(87)90079-6
• 发表时间: 1987
• 期刊: Biochimica et biophysica acta
• 作者: Bock,JL;Wenz,B;Gupta,RK
• 通讯作者: Gupta,RK

On the noninvasive measurement of intracellular free magnesium by 31P NMR spectroscopy.

利用 31P NMR 光谱法无创测量细胞内游离镁。

• 发表时间: 1983
• 期刊: Physiological chemistry and physics and medical NMR
• 作者: Gupta,RK;Gupta,P;Yushok,WD;Rose,ZB
• 通讯作者: Rose,ZB

23Na NMR spectroscopy of proximal tubule suspensions.

近端小管悬浮液的 23Na NMR 光谱。

• DOI: 10.1038/ki.1986.61
• 发表时间: 1986
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: Kumar,AM;Spitzer,A;Gupta,RK
• 通讯作者: Gupta,RK