9 4 TESLA WIDE BORE MULTI-NUCLEAR NMR SPECTROMETER

一台最先进的宽口径多核核磁共振仪 在9.4特斯拉的磁场下，需要9个主要的 来自六个不同部门的用户（生理学和生物物理学， 儿科学、医学、生物化学、分子药理学和 发育生物学和癌症）和24个未成年人用户。 各次主要 Gupta博士建议对金属离子（Na+，K+，Ca2+， Mg2+）在各种分离的细胞和组织中。 他寻求 了解金属离子在细胞增殖中的作用， 分化，体积调节和激素控制的各种 细胞过程，并研究细胞内的紊乱 离子浓度灌注大鼠肝脏研究离子和 伴随肝再生的代谢变化。 约翰罗森博士 和弗兰克·沙恩将使用宽口径核磁共振进行他们的研究， 铅的毒性机制，特别是对细胞的影响 骨细胞中的钙稳态。 詹姆斯·朔伊尔博士将使用 多核核磁共振灌注大鼠心脏研究的脆弱性 导致心肌缺血而斯皮策医生会利用 磷NMR检查肾磷酸盐转运过程中 发展 Fabry博士将利用高分辨率NMR研究 疟原虫红细胞的代谢以及 结合表面线圈，研究镰状动物模型 细胞血管闭塞。 Vern Schramm博士将利用NMR进行 阐明了催化机理和过渡态结构 以及生物素在丙酮酸水解酶中的作用 羧化酶反应 布鲁尔博士将继续追踪 核磁共振在糖肽研究中的应用 寡糖与伴刀豆球蛋白A的相互作用。 阿特金森医生 使用一维和二维高场质子NMR光谱， 转化酶复合糖结构解析 互补组胞吐突变。 所有提出的 主要用户和次要用户将从 大口径多核核磁共振仪的可用性。

A state-of-the-art wide-bore multinuclear NMR instrument operating at a magnetic field of 9.4 Tesla is needed for a group of 9 major users from six different departments (Physiology & Biophysics, Pediatrics, Medicine, Biochemistry, Molecular Pharmacology and Developmental Biology & Cancer) and 24 minor users. Of the major users, Dr. Gupta proposes NMR studies of metal ions (Na+, K+, Ca2+, Mg2+) in a variety of isolated cells and tissues. He seeks to understand involvement of metal ions in cellular proliferation, differentiation, volume regulation, and hormonal control of various cellular processes, and to study the derangement of intracellular ionic concentrations in perfused rat liver to study ionic and metabolic changes accompanying liver regeneration. Drs. John Rosen and Frank Schanne will use wide-bore NMR for their studies on the mechanism of lead toxicity, especially its effect on cellular calcium homeostasis in bone cells. Dr. James Scheuer will use multinuclear NMR of perfused rat heart to study vulnerability of the heart to myocardial ischemia, while Dr. Spitzer will exploit phosphorous NMR to examine renal phosphate transport during development. Dr. Fabry will utilize high resolution NMR to study metabolism of malaria parasitized red cell as well as, in combination with surface coils, to study an animal model for sickle cell vaso-occlusion. Dr. Vern Schramm will utilize NMR for elucidating the catalytic mechanism and transition state structures of glycohydrolases and the role of biotin in the pyruvate carboxylase reaction. Dr. Brewer will continue to pursue applications of NMR in the study of glycopeptide and oligosaccharide interactions with concanavalin A. Dr. Atkinson uses l-D as well as 2-D high field proton NMR spectroscopy of elucidation of complex carbohydrate structure on invertase complementation groups mutant in exocytosis. All of the proposed major and minor users will derive significant benefit from the availability of a wide-bore multinuclear NMR instrument.