REICHERT-JUNG CRYOFRACT 190 WITH CRYOBLOCK I

REICHERT-JUNG CRYOFRACT 190 与 CRYOBLOCK I

• 批准号: 3520112
• 负责人: LARRY F LEMANSKI
• 金额: $ 12.4万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520112
• 关键词: biomedical equipment purchase; cell biology; cryoscience; freeze etching; molecular biology

There is substantial interest at the SUNY Health Science Center, Syracuse, in research on various aspects of cellular and molecular biology. Several NIH funded programs currently have substantial need for a state-of-the-art freeze-fracturing machine such as the Reichert-Jung Cryofract 190 with Cryoblock (or equivalent). In addition, several recently-appointed faculty members not yet funded will have need for this type of instrument in the near future. Initially, the major users of the instrument have their primary academic appointments in six different departments and represent three university campuses. The freeze-fracture unit will be housed in the centrally-located Department of Anatomy and Cell Biology electron Microscopy Suite where a full-time technically trained individual will oversee its maintenance and operation. The funded projects which will initially require the use of the freeze fracture instrument include: three-dimensional localization of contractile/cytoskeletal/membrane proteins in developing myocytes; gap junctions in intestinal smooth muscle; structure of the cochlear hair cells; information processing by the parietal eye; gap junction/membrane relationships; electrical coupling and gap junction formation; membrane vesicles-structure and function; dynein-ATPase morphology; contractile proteins and membrane relationships in inner hair cells; localization of calcium-calmodulin dependent kinase on developing neurites; and embryological development of gap junctions in developing cells. Moreover, it is anticipated that several new incoming faculty members in the Department of Anatomy and Cell Biology and Pharmacology will become major users of the instrument. Thus, there is clearly an urgent, current need for a new freeze fracture unit and such an instrument will be heavily used for many years to come.

纽约州立大学健康科学中心对此很感兴趣， 锡拉丘兹，在细胞和分子的各个方面的研究 生物学 一些NIH资助的项目目前有大量的 需要一个国家的最先进的冷冻破碎机，如 Reichert-Jung Cryofract 190（带Cryoblock）（或同等产品）。 在 此外，最近任命的几名教师还没有 资金将需要这种类型的仪器在不久的将来， 未来 最初，仪器的主要用户有他们的 六个不同系的主要学术任命， 代表了三个大学校园。 冷冻断裂装置 将被安置在位于市中心的解剖学系 和细胞生物学电子显微镜套件， 受过技术培训的个人将监督其维护， 操作 最初需要使用的资助项目 冷冻断裂仪的主要功能包括： 收缩/细胞骨架/膜蛋白的定位 肠平滑肌缝隙连接; 耳蜗毛细胞的结构;耳蜗毛细胞的信息处理 顶眼;缝隙连接/膜关系;电 偶联和缝隙连接形成;膜囊泡结构;细胞膜 和功能;动力蛋白-ATP酶形态学;收缩蛋白和 内毛细胞的膜关系; 发育中的神经突上的钙-钙调蛋白依赖性激酶;和 发育细胞中缝隙连接的胚胎发育。 此外，预计将有几名新的教员 解剖学和细胞生物学系的成员， 药理学将成为该仪器的主要用户。 因此，在本发明中， 很明显，目前迫切需要一种新的冷冻断裂法， 这种仪器将被大量使用多年， 来了