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Thermophoretic manipulation of biocompatible soft materials properties in microfluidic devices

微流体装置中生物相容性软材料特性的热泳操控

基本信息

批准号：
EP/R004382/1
负责人：
Daniele Vigolo
金额：
$ 12.67万
依托单位：
University of Birmingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FR004382%2F1
关键词：
Thermophoretic manipulation biocompatible soft materials

项目摘要

In recent years microfluidics has already proven incredibly useful in tackling biological problems, from single cell analyses up to organ reproduced on-a-chip. Current biological approaches require in-vitro experiments to test preliminary hypotheses: often these synthetic environments share little to nothing with the actual environment they are trying to mimic and thus the subsequent in-vivo experiments become the only way to actually perform the necessary tests. Being able to modify the biocompatible material's properties at the length scale typical of a single cell, will open up a new set of tools enabling to perform realistic biological tests that can save time in the process of understanding the mechanisms of the development of a disease and the search for an effective cure. This is what motivates this proposal. Current challenges in the field of tissue engineering are strongly limited by the availability of functionalised biocompatible materials that can provide the optimal substrate or the scaffold to be used to guide the growth of cells. This proposal aims to exploit an innovative way to locally manipulate the mechanical, optical and rheological properties of a soft material at the micron scale, and thus develop a new class of functionalised materials. This project will exploit thermophoresis in a microfluidic environment to induce a concentration gradient in a polymeric solution by imposing a highly localised temperature gradient. The concentration gradient will then translate into a gradient of properties that I will tune and manipulate. This will enable me to generate biocompatible materials with unprecedented control over their mechanical properties where to study the proliferation and differentiation of cells, and shine light on the mechanisms of cancer invasion. One of the main objectives of this proposal is the extension of the study to biocompatible substrate where the mechanical properties are dynamically tunable. This will permit me to investigate the response to a change in external stimuli of a growing tissue and to model the dynamics of wound healing, where the characteristics of the substrate are changing while the epithelium is growing. Additionally, I will investigate the growth of cells on fibre-like and more complex structures substrates, in order to induce control the proliferation, growth and even differentiation of behaviour of specific cell lines.

近年来，微流体技术已被证明在解决生物问题方面非常有用，从单细胞分析到芯片上的器官复制。当前的生物学方法需要体外实验来测试初步假设：通常这些合成环境与它们试图模仿的实际环境几乎没有什么共同之处，因此随后的体内实验成为实际执行必要测试的唯一方法。能够在单细胞典型的长度尺度上修改生物相容性材料的特性，将开辟一套新的工具，能够进行现实的生物测试，可以在了解疾病发展机制和寻找有效治疗方法的过程中节省时间。这就是该提案的动机。当前组织工程领域的挑战受到功能化生物相容性材料的可用性的严重限制，这些材料可以提供用于引导细胞生长的最佳基质或支架。该提案旨在开发一种创新方法，在微米级局部操纵软材料的机械、光学和流变特性，从而开发一类新型功能化材料。该项目将利用微流体环境中的热泳，通过施加高度局部的温度梯度来诱导聚合物溶液中的浓度梯度。然后，浓度梯度将转化为我将调整和操纵的属性梯度。这将使我能够生产出生物相容性材料，对其机械性能进行前所未有的控制，以研究细胞的增殖和分化，并揭示癌症侵袭的机制。该提案的主要目标之一是将研究扩展到机械性能可动态调节的生物相容性基材。这将使我能够研究生长组织对外部刺激变化的反应，并模拟伤口愈合的动态，其中基质的特性在上皮生长时发生变化。此外，我将研究细胞在纤维状和更复杂结构基底上的生长，以诱导控制特定细胞系的增殖、生长甚至分化行为。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Effect of Bacteria on the Stability of Microfluidic-Generated Water-in-Oil Droplet.

细菌对微流体生成的水滴稳定性的影响。

DOI：
10.3390/mi13122067
发表时间：
2022-11-25
期刊：
MICROMACHINES
影响因子：
3.4
作者：
Isa, Nur Suaidah Mohd;El Kadri, Hani;Vigolo, Daniele;Gkatzionis, Konstantinos
通讯作者：
Gkatzionis, Konstantinos

Optimisation of bacterial release from a stable microfluidic-generated water-in-oil-in-water emulsion.